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Microorganisms
Volume 10
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10.3390/microorganisms10061141
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Article

Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment

by
Calmes Ursain Bouaka Tsakeng
1,2,
Tito Tresor Melachio Tanekou
1,3,*,
Steve Feudjio Soffack
4,
Inaki Tirados
5,
Cedrique Noutchih
1,
Flobert Njiokou
1,4,
Jude Daiga Bigoga
2 and
Charles Sinclair Wondji
1,5
1
Centre for Research in Infectious Diseases (CRID), Yaoundé P.O. Box 13591, Cameroon
2
Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé P.O. Box 812, Cameroon
3
Department of Biological Sciences, Faculty of Science, University of Bamenda, Bamenda P.O. Box 39, Cameroon
4
Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, Yaoundé P.O. Box 812, Cameroon
5
Department of Vector Biology, Liverpool School of Tropical Medicine Pembroke Place, Liverpool L3 5QA, UK
*
Author to whom correspondence should be addressed.
Microorganisms 2022, 10(6), 1141; https://doi.org/10.3390/microorganisms10061141
Submission received: 2 May 2022 / Accepted: 30 May 2022 / Published: 31 May 2022
(This article belongs to the Special Issue Microbiota in Insects)
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Abstract

The tsetse flies, biological vectors of African trypanosomes, harbour a variety of bacteria involved in their vector competence that may help in developing novel vector control tools. This study provides an inventory of tsetse bacterial communities in Cameroon and explores their possible associations with trypanosome establishment in Glossina palpalis palpalis. High throughput sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene, with subsequent metagenomic, multivariate, and association analyses, were used to investigate the levels and patterns of microbial diversity in four tsetse species. Overall, 31 bacterial genera and four phyla were identified. The primary symbiont Wigglesworthia dominated almost all the samples, with an overall relative abundance of 47.29%, and seemed to be replaced by Serratia or Burkholderia in some G. tachinoides flies. Globally, significant differences were observed in the microbiome diversity and composition among tsetse species and between teneral and non-teneral flies, or between flies displaying or not displaying mature trypanosome infections. In addition, differential abundance testing showed some OTUs, or some bacteria taxa, associated with trypanosome maturation in tsetse flies. These bacteria could be further investigated for an understanding of their mechanism of action and alternatively, transformed and used to block trypanosome development in tsetse flies.
Keywords: tsetse flies; trypanosomes; microbiome; vector competence; vector control tsetse flies; trypanosomes; microbiome; vector competence; vector control

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MDPI and ACS Style

Tsakeng, C.U.B.; Tanekou, T.T.M.; Soffack, S.F.; Tirados, I.; Noutchih, C.; Njiokou, F.; Bigoga, J.D.; Wondji, C.S. Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment. Microorganisms 2022, 10, 1141. https://doi.org/10.3390/microorganisms10061141

AMA Style

Tsakeng CUB, Tanekou TTM, Soffack SF, Tirados I, Noutchih C, Njiokou F, Bigoga JD, Wondji CS. Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment. Microorganisms. 2022; 10(6):1141. https://doi.org/10.3390/microorganisms10061141

Chicago/Turabian Style

Tsakeng, Calmes Ursain Bouaka, Tito Tresor Melachio Tanekou, Steve Feudjio Soffack, Inaki Tirados, Cedrique Noutchih, Flobert Njiokou, Jude Daiga Bigoga, and Charles Sinclair Wondji. 2022. "Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment" Microorganisms 10, no. 6: 1141. https://doi.org/10.3390/microorganisms10061141

APA Style

Tsakeng, C. U. B., Tanekou, T. T. M., Soffack, S. F., Tirados, I., Noutchih, C., Njiokou, F., Bigoga, J. D., & Wondji, C. S. (2022). Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment. Microorganisms, 10(6), 1141. https://doi.org/10.3390/microorganisms10061141

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