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Volume 10
Issue 2
10.3390/microorganisms10020425
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Article

Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit

by
Md. Arshad Ali
1,
Jinyan Luo
2,
Temoor Ahmed
1,
Jiannan Zhang
1,
Ting Xie
1,
Dejiang Dai
3,*,
Jingyong Jiang
4,
Jie Zhu
5,
Sabry Hassan
6,
Jamal A. Alorabi
6,
Bin Li
1 and
Qianli An
1,*
1
State Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, College of Agricultural and Biotechnology, Zhejiang University, Hangzhou 310058, China
2
Department of Plant Quarantine, Shanghai Extension and Service Center of Agriculture Technology, Shanghai 201103, China
3
Station for the Plant Protection & Quarantine and Control of Agrochemicals Zhejiang Province, Hangzhou 310004, China
4
Taizhou Academy of Agricultural Sciences, Linhai 317000, China
5
Wenzhou Station of Plant Protection, Soils and Fertilizers, Wenzhou 325000, China
6
Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
*
Authors to whom correspondence should be addressed.
Microorganisms 2022, 10(2), 425; https://doi.org/10.3390/microorganisms10020425
Submission received: 15 December 2021 / Revised: 9 February 2022 / Accepted: 10 February 2022 / Published: 12 February 2022
(This article belongs to the Special Issue Plant-Associated Pseudomonads)
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Abstract

Kiwifruit worldwide suffers from the devastating diseases of bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) and gray mold caused by Botrytis cinerea. Here, an endophytic bacterium XL17 isolated from a rape crown gall was screened out for its potent antagonistic activities against Psa and B. cinerea. Strain XL17 and its cell-free culture filtrate (CF) inhibited the growth of Psa and B. cinerea, Psa-associated leaf necrosis, and B. cinerea-associated kiwifruit necrosis. Electron microscopy showed that XL17 CF could damage the cell structures of Psa and B. cinerea. Genome-based taxonomy revealed that strain XL17 belongs to Pseudomonas bijieensis within the P. corrugata subgroup of the P. fluorescens species complex. Among the P. corrugata subgroup containing 31 genomospecies, the presence of the phl operon responsible for the biosynthesis of the phenolic polyketide 2,4-diacetylphloroglucinol (DAPG) and the absence of the lipopeptide/quorum sensing island can serve as the genetic marker for the determination of a plant-protection life style. HPLC detected DAPG in extracts from XL17 CF. MALDI-TOF-MS analysis revealed that strain XL17 produced cyclic lipopeptides of the viscosin family and orfamide family. Together, phenotypic, genomic, and metabolic analyses identified that P. bijieensis XL17 producing DAPG and cyclic lipopeptides can be used to control bacterial canker and gray mold pathogens of kiwifruit.
Keywords: biocontrol; Pseudomonas syringae pv. actinidiae; Botrytis cinerea; DAPG; cyclic lipopeptides biocontrol; Pseudomonas syringae pv. actinidiae; Botrytis cinerea; DAPG; cyclic lipopeptides

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MDPI and ACS Style

Ali, M.A.; Luo, J.; Ahmed, T.; Zhang, J.; Xie, T.; Dai, D.; Jiang, J.; Zhu, J.; Hassan, S.; Alorabi, J.A.; et al. Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit. Microorganisms 2022, 10, 425. https://doi.org/10.3390/microorganisms10020425

AMA Style

Ali MA, Luo J, Ahmed T, Zhang J, Xie T, Dai D, Jiang J, Zhu J, Hassan S, Alorabi JA, et al. Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit. Microorganisms. 2022; 10(2):425. https://doi.org/10.3390/microorganisms10020425

Chicago/Turabian Style

Ali, Md. Arshad, Jinyan Luo, Temoor Ahmed, Jiannan Zhang, Ting Xie, Dejiang Dai, Jingyong Jiang, Jie Zhu, Sabry Hassan, Jamal A. Alorabi, and et al. 2022. "Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit" Microorganisms 10, no. 2: 425. https://doi.org/10.3390/microorganisms10020425

APA Style

Ali, M. A., Luo, J., Ahmed, T., Zhang, J., Xie, T., Dai, D., Jiang, J., Zhu, J., Hassan, S., Alorabi, J. A., Li, B., & An, Q. (2022). Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit. Microorganisms, 10(2), 425. https://doi.org/10.3390/microorganisms10020425

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