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Microorganisms
Volume 10
Issue 2
10.3390/microorganisms10020418
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Article

Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling

by
Thu Huynh
1,2,3,
Mónika Vörös
1,
Orsolya Kedves
1,
Adiyadolgor Turbat
1,
György Sipos
4,
Balázs Leitgeb
5,
László Kredics
1,
Csaba Vágvölgyi
1 and
András Szekeres
1,*
1
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary
2
Department of Biotechnology, Faculty of Chemical Engineering, Ho Chi Minh University of Technology (HCMUT), 268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City 72607, Vietnam
3
Vietnam National University Ho Chi Minh City, Linh Trung Ward, Thu Duc District, Ho Chi Minh City 71351, Vietnam
4
Functional Genomics and Bioinformatics Group, Research Center for Forestry and Wood Industry, University of Sopron, Bajcsy-Zsilinszky Str. 4, H-9400 Sopron, Hungary
5
Institute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Microorganisms 2022, 10(2), 418; https://doi.org/10.3390/microorganisms10020418
Submission received: 31 December 2021 / Revised: 3 February 2022 / Accepted: 8 February 2022 / Published: 11 February 2022
(This article belongs to the Section Microbial Biotechnology)
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Abstract

(1) Background: Bacillus velezensis and Bacillus amyloliquefaciens are closely related members of the “operational group B. amyloliquefaciens”, a taxonomical unit above species level within the ”Bacillus subtilis species complex”. They have similar morphological, physiological, biochemical, phenotypic, and phylogenetic characteristics. Thus, separating these two taxa from each another has proven to be difficult to implement and could not be pushed easily into the line of routine analyses. (2) Methods: The aim of this study was to determine whether whole FAME profiling could be used to distinguish between these two species, using both type strains and environmental isolates. Initially, the classification was determined by partial sequences of the gyrA and rpoB genes and the classified isolates and type strains were considered as samples to develop the identification method, based on FAME profiles. (3) Results: The dissimilarities in 16:0, 17:0 iso, and 17:0 FA components have drawn a distinction between the two species and minor differences in FA 14:0, 15:0 iso, and 16:0 iso were also visible. The statistical analysis of the FA profiles confirmed that the two taxa can be distinguished into two separate groups, where the isolates are identified without misreading. (4) Conclusions: Our study proposes that the developed easy and fast-automated identification tool based on cellular FA profiles can be routinely applied to distinguish B. velezensis and B. amyloliquefaciens.
Keywords: Bacillus taxonomy; Bacillus velezensis; Bacillus amyloliquefaciens; fatty acid profiling; chemotaxonomy Bacillus taxonomy; Bacillus velezensis; Bacillus amyloliquefaciens; fatty acid profiling; chemotaxonomy

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MDPI and ACS Style

Huynh, T.; Vörös, M.; Kedves, O.; Turbat, A.; Sipos, G.; Leitgeb, B.; Kredics, L.; Vágvölgyi, C.; Szekeres, A. Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling. Microorganisms 2022, 10, 418. https://doi.org/10.3390/microorganisms10020418

AMA Style

Huynh T, Vörös M, Kedves O, Turbat A, Sipos G, Leitgeb B, Kredics L, Vágvölgyi C, Szekeres A. Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling. Microorganisms. 2022; 10(2):418. https://doi.org/10.3390/microorganisms10020418

Chicago/Turabian Style

Huynh, Thu, Mónika Vörös, Orsolya Kedves, Adiyadolgor Turbat, György Sipos, Balázs Leitgeb, László Kredics, Csaba Vágvölgyi, and András Szekeres. 2022. "Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling" Microorganisms 10, no. 2: 418. https://doi.org/10.3390/microorganisms10020418

APA Style

Huynh, T., Vörös, M., Kedves, O., Turbat, A., Sipos, G., Leitgeb, B., Kredics, L., Vágvölgyi, C., & Szekeres, A. (2022). Discrimination between the Two Closely Related Species of the Operational Group B. amyloliquefaciens Based on Whole-Cell Fatty Acid Profiling. Microorganisms, 10(2), 418. https://doi.org/10.3390/microorganisms10020418

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