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Article

Monitoring Mycoplasma bovis Diversity and Antimicrobial Susceptibility in Calf Feedlots Undergoing a Respiratory Disease Outbreak

1
UMR Mycoplasmoses des Ruminants, VetAgro Sup, Université de Lyon, 69280 Marcy-l’Etoile, France
2
UMR Mycoplasmoses des Ruminants, ANSES Laboratoire de Lyon, Université de Lyon, 69364 Lyon CEDEX 07, France
*
Author to whom correspondence should be addressed.
Current Address: Cancer Research Centre of Lyon, 69008 Lyon, France.
Current Address: Mylan Laboratories SAS, 01400 Châtillon-sur-Chalaronne, France.
Pathogens 2020, 9(7), 593; https://doi.org/10.3390/pathogens9070593
Received: 30 June 2020 / Revised: 10 July 2020 / Accepted: 11 July 2020 / Published: 21 July 2020
Bovine respiratory diseases (BRD) are widespread in veal calf feedlots. Several pathogens are implicated, both viruses and bacteria, one of which, Mycoplasma bovis, is under-researched. This worldwide-distributed bacterium has been shown to be highly resistant in vitro to the main antimicrobials used to treat BRD. Our objective was to monitor the relative prevalence of M. bovis during BRD episodes, its diversity, and its resistance phenotype in relation to antimicrobial use. For this purpose, a two-year longitudinal follow-up of 25 feedlots was organized and 537 nasal swabs were collected on 358 veal calves at their arrival in the lot, at the BRD peak and 4 weeks after collective antimicrobial treatments. The presence of M. bovis was assessed by real-time PCR and culture. The clones isolated were then subtyped (polC subtyping and PFGE analysis), and their susceptibility to five antimicrobials was determined. The course of the disease and the antimicrobials used had no influence on the genetic diversity of the M. bovis strains: The subtype distribution was the same throughout the BRD episode and similar to that already described in France, with a major narrowly-variable subtype circulating, st2. The same conclusion holds for antimicrobial resistance (AMR) phenotypes: All the clones were already multiresistant to the main antimicrobials used (except for fluoroquinolones) prior to any treatments. By contrast, changes of AMR phenotypes could be suspected for Pasteurellaceae in two cases in relation to the treatments registered. View Full-Text
Keywords: Mycoplasma bovis; antimicrobial resistance; Bovine Respiratory Disease; genetic diversity Mycoplasma bovis; antimicrobial resistance; Bovine Respiratory Disease; genetic diversity
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MDPI and ACS Style

Becker, C.A.M.; Ambroset, C.; Huleux, A.; Vialatte, A.; Colin, A.; Tricot, A.; Arcangioli, M.-A.; Tardy, F. Monitoring Mycoplasma bovis Diversity and Antimicrobial Susceptibility in Calf Feedlots Undergoing a Respiratory Disease Outbreak. Pathogens 2020, 9, 593. https://doi.org/10.3390/pathogens9070593

AMA Style

Becker CAM, Ambroset C, Huleux A, Vialatte A, Colin A, Tricot A, Arcangioli M-A, Tardy F. Monitoring Mycoplasma bovis Diversity and Antimicrobial Susceptibility in Calf Feedlots Undergoing a Respiratory Disease Outbreak. Pathogens. 2020; 9(7):593. https://doi.org/10.3390/pathogens9070593

Chicago/Turabian Style

Becker, Claire A.M.; Ambroset, Chloé; Huleux, Anthéa; Vialatte, Angélique; Colin, Adélie; Tricot, Agnès; Arcangioli, Marie-Anne; Tardy, Florence. 2020. "Monitoring Mycoplasma bovis Diversity and Antimicrobial Susceptibility in Calf Feedlots Undergoing a Respiratory Disease Outbreak" Pathogens 9, no. 7: 593. https://doi.org/10.3390/pathogens9070593

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