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Prevalence of the Puumala orthohantavirus Strains in the Pre-Kama Area of the Republic of Tatarstan, Russia

Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment

Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, 17493 Greifswald-Insel Riems, Germany
Cheplapharm Arzneimittel-GmbH, 17489 Greifswald, Germany
Institute for Medical Immunology, Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, 13353 Berlin, Germany
Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Horticulture and Forests, Vertebrate Research, 48161 Münster, Germany
Institute of Ecology and Evolution, University of Bern, 3012 Bern, Switzerland
Author to whom correspondence should be addressed.
Current address: Institute of Biochemistry and Biology, Animal Ecology, University of Potsdam, 14469 Potsdam, Germany.
Pathogens 2020, 9(7), 548;
Received: 26 May 2020 / Revised: 24 June 2020 / Accepted: 28 June 2020 / Published: 8 July 2020
(This article belongs to the Special Issue Hantavirus Infections)
The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolution and test for spillover infections in sympatric rodent species, during 2010–2014, 883 bank voles, 357 yellow-necked mice (Apodemus flavicollis), 62 wood mice (A. sylvaticus), 149 common voles (Microtus arvalis) and 8 field voles (M. agrestis) were collected in Baden-Wuerttemberg and North Rhine-Westphalia, Germany. In total, 27.9% and 22.3% of bank voles were positive for PUUV-reactive antibodies and PUUV-specific RNA, respectively. One of eight field voles was PUUV RNA-positive, indicating a spillover infection, but none of the other species showed evidence of PUUV infection. Phylogenetic and isolation-by-distance analyses demonstrated a spatial clustering of PUUV S segment sequences. In the hantavirus outbreak years 2010 and 2012, PUUV RNA prevalence was higher in our study regions compared to non-outbreak years 2011, 2013 and 2014. NSs amino acid and nucleotide sequence types showed temporal and/or local variation, whereas the N protein was highly conserved in the NSs overlapping region and, to a lower rate, in the N alone coding part. View Full-Text
Keywords: hantavirus; bank vole; evolution; N protein; NSs protein; S segment hantavirus; bank vole; evolution; N protein; NSs protein; S segment
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MDPI and ACS Style

Binder, F.; Ryll, R.; Drewes, S.; Jagdmann, S.; Reil, D.; Hiltbrunner, M.; Rosenfeld, U.M.; Imholt, C.; Jacob, J.; Heckel, G.; Ulrich, R.G. Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment. Pathogens 2020, 9, 548.

AMA Style

Binder F, Ryll R, Drewes S, Jagdmann S, Reil D, Hiltbrunner M, Rosenfeld UM, Imholt C, Jacob J, Heckel G, Ulrich RG. Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment. Pathogens. 2020; 9(7):548.

Chicago/Turabian Style

Binder, Florian, René Ryll, Stephan Drewes, Sandra Jagdmann, Daniela Reil, Melanie Hiltbrunner, Ulrike M. Rosenfeld, Christian Imholt, Jens Jacob, Gerald Heckel, and Rainer G. Ulrich. 2020. "Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment" Pathogens 9, no. 7: 548.

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