Next Article in Journal
A Cross-Sectional Study of Seroprevalence of Strongyloidiasis in Pregnant Women (Peruvian Amazon Basin)
Previous Article in Journal
Evaluation of Volatile Organic Compounds Obtained from Breath and Feces to Detect Mycobacterium tuberculosis Complex in Wild Boar (Sus scrofa) in Doñana National Park, Spain
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pathogens
Volume 9
Issue 5
10.3390/pathogens9050347
pathogens-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece

by
Konstantinos Arsenopoulos
1,
Styliani Minoudi
2,
Isaia Symeonidou
1,
Alexandros Triantafyllidis
2,
Angeliki I. Katsafadou
3,
Daphne T. Lianou
3,
George C. Fthenakis
3 and
Elias Papadopoulos
1,*
1
Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
2
Laboratory of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
3
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(5), 347; https://doi.org/10.3390/pathogens9050347
Submission received: 4 April 2020 / Revised: 29 April 2020 / Accepted: 30 April 2020 / Published: 3 May 2020
(This article belongs to the Section Animal Pathogens)
Browse Figures
Versions Notes

Abstract

The study investigated the presence of resistance to benzimidazoles in Haemonchus contortus helminths from ruminant species in Greece through the detection of the Phe/Tyr polymorphism in the amino acid at position 200 of the β-tubulin protein. In total, 288 adult female H. contortus helminths collected from the abomasum of various ruminant animals in Greece were tested. Of these, 96 were collected from sheep, 96 from goats, 48 from cattle, and 48 from buffaloes. The frequencies of the homozygous and heterozygous resistant genotypes at the position 200 of the β-tubulin gene of helminths recovered from sheep were 96.9% and 3.1%, respectively. The frequencies of the homozygous and heterozygous resistant genotypes, respectively, were 100.0% and 0.0% in helminths from goats, 25.0% and 75.0% in helminths from cattle and 8.3% and 91.7% in helminths from buffaloes. In all parasitic populations, no homozygous susceptible genotypes were detected. The present study highlighted, for the first time, the emergence of benzimidazole-resistant H. contortus in goats, cattle, and buffaloes in Greece, using an allele-specific PCR. It is postulated that benzimidazole-resistant alleles were transferred from sheep or goats to cattle and buffaloes at the commonly grazing pastures in Greece.
Keywords: allele-specific PCR; benzimidazole resistance; buffalo; cattle; goat; Haemonchus contortus; sheep allele-specific PCR; benzimidazole resistance; buffalo; cattle; goat; Haemonchus contortus; sheep

Share and Cite

MDPI and ACS Style

Arsenopoulos, K.; Minoudi, S.; Symeonidou, I.; Triantafyllidis, A.; Katsafadou, A.I.; Lianou, D.T.; Fthenakis, G.C.; Papadopoulos, E. Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece. Pathogens 2020, 9, 347. https://doi.org/10.3390/pathogens9050347

AMA Style

Arsenopoulos K, Minoudi S, Symeonidou I, Triantafyllidis A, Katsafadou AI, Lianou DT, Fthenakis GC, Papadopoulos E. Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece. Pathogens. 2020; 9(5):347. https://doi.org/10.3390/pathogens9050347

Chicago/Turabian Style

Arsenopoulos, Konstantinos, Styliani Minoudi, Isaia Symeonidou, Alexandros Triantafyllidis, Angeliki I. Katsafadou, Daphne T. Lianou, George C. Fthenakis, and Elias Papadopoulos. 2020. "Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece" Pathogens 9, no. 5: 347. https://doi.org/10.3390/pathogens9050347

APA Style

Arsenopoulos, K., Minoudi, S., Symeonidou, I., Triantafyllidis, A., Katsafadou, A. I., Lianou, D. T., Fthenakis, G. C., & Papadopoulos, E. (2020). Frequency of Resistance to Benzimidazoles of Haemonchus contortus Helminths from Dairy Sheep, Goats, Cattle and Buffaloes in Greece. Pathogens, 9(5), 347. https://doi.org/10.3390/pathogens9050347

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop