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Open AccessArticle

Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis

1
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea
2
Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, Korea
3
Foot-and-Mouth Disease Division, Animal and Plant Quarantine Agency, Gyongsangbuk-do 39660, Korea
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 301; https://doi.org/10.3390/pathogens8040301
Received: 12 November 2019 / Revised: 13 December 2019 / Accepted: 15 December 2019 / Published: 17 December 2019
Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A is one of the most common causes of FMD outbreaks among the seven FMDV serotypes, and its serological diagnosis is therefore important to confirm FMDV type A infection and to determine FMD vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific to FMDV type A via hybridoma systems using an inactivated FMDV type A (A22/Iraq/1964) and found 4 monoclones (#29, #106, #108, and #109) with high binding reactivity to FMDV type A among 594 primary clones. In particular, the #106 mAb had a higher binding reactivity to the inactivated FMDV type A than the other mAbs and a commercial mAb. Moreover, the #106 mAb showed no cross-reactivity to inactivated FMDV type South African territories 1, 2, and 3, and low reactivity to inactivated FMDV type O (O1 Manisa). Importantly, the solid-phase competitive ELISA (SPCE) using horseradish peroxidase (HRP)-conjugated #106 mAb detected FMDV type A-specific Abs in sera from FMD type A-vaccinated cattle more effectively than a commercial SPCE. These results suggest that the newly developed FMDV type A-specific mAb might be useful for diagnostic approaches for detecting Abs against FMDV type A. View Full-Text
Keywords: foot-and-mouth disease; type A; structural protein; monoclonal antibody; solid-phase competitive ELISA; sensitivity foot-and-mouth disease; type A; structural protein; monoclonal antibody; solid-phase competitive ELISA; sensitivity
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Nguyen, Q.T.; Yang, J.; Byun, J.-W.; Pyo, H.M.; Park, M.-Y.; Ku, B.K.; Nah, J.; Ryoo, S.; Wee, S.-H.; Choi, K.-S.; Poo, H. Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis. Pathogens 2019, 8, 301.

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