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Aggregatibacter Actinomycetemcomitans: Clinical Significance of a Pathobiont Subjected to Ample Changes in Classification and Nomenclature
Open AccessArticle

Whole Genome Sequencing of Aggregatibacter actinomycetemcomitans Cultured from Blood Stream Infections Reveals Three Major Phylogenetic Groups Including a Novel Lineage Expressing Serotype a Membrane O Polysaccharide

1
Department of Clinical Microbiology, Aarhus University Hospital, DK-8200 Aarhus N, Denmark
2
Bioinformatics Research Centre, Aarhus University, DK-8000 Aarhus C, Denmark
3
Department of Clinical Biochemistry and Pharmacology, Odense University hospital, DK-5000 Odense C, Denmark
4
Department of Dentistry, Aarhus University, DK-8000 Aarhus C, Denmark
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 256; https://doi.org/10.3390/pathogens8040256
Received: 1 October 2019 / Revised: 18 November 2019 / Accepted: 19 November 2019 / Published: 22 November 2019
Twenty-nine strains of Aggregatibacter actinomycetemcomitans cultured from blood stream infections in Denmark were characterised. Serotyping was unremarkable, with almost equal proportions of the three major types plus a single serotype e strain. Whole genome sequencing positioned the serotype e strain outside the species boundary; moreover, one of the serotype a strains was unrelated to other strains of the major serotypes and to deposited sequences in the public databases. We identified five additional strains of this type in our collections. The particularity of the group was corroborated by phylogenetic analysis of concatenated core genes present in all strains of the species, and by uneven distribution of accessory genes only present in a subset of strains. Currently, the most accurate depiction of A. actinomycetemcomitans is a division into three lineages that differ in genomic content and competence for transformation. The clinical relevance of the different lineages is not known, and even strains excluded from the species sensu stricto can cause serious human infections. Serotyping is insufficient for characterisation, and serotypes a and e are not confined to specific lineages. View Full-Text
Keywords: taxonomy; core and accessory genes; average nucleotide identity; principal component analysis taxonomy; core and accessory genes; average nucleotide identity; principal component analysis
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Nedergaard, S.; Kobel, C.M.; Nielsen, M.B.; Møller, R.T.; Jensen, A.B.; Nørskov-Lauritsen, N. Whole Genome Sequencing of Aggregatibacter actinomycetemcomitans Cultured from Blood Stream Infections Reveals Three Major Phylogenetic Groups Including a Novel Lineage Expressing Serotype a Membrane O Polysaccharide. Pathogens 2019, 8, 256.

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