Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR
by
Patharapol Piamsomboon 1,†
, Seong-Kyoon Choi 2,3,†, Bambang Hanggono 4, Yani Lestari Nuraini 4, Fatma Wati 4, Kathy F. J. Tang 5, Song Park 2, Dongmi Kwak 6, Man Hee Rhee 6, Jee Eun Han 6,* and Ji Hyung Kim 7,*
Patharapol Piamsomboon 1,†, Seong-Kyoon Choi 2,3,†, Bambang Hanggono 4, Yani Lestari Nuraini 4, Fatma Wati 4, Kathy F. J. Tang 5, Song Park 2, Dongmi Kwak 6, Man Hee Rhee 6, Jee Eun Han 6,* and Ji Hyung Kim 7,*
1
Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
2
Core Protein Resources Center, DGIST, Daegu 42988, Korea
3
Division of Biotechnology, DGIST, Daegu 42988, Korea
4
Fish Health and Environmental Laboratory, Brackishwater Aquaculture Development Center, Situbondo 68351, Indonesia
5
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
6
College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
7
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
*
Authors to whom correspondence should be addressed.
†
These authors equally contributed in this work.
Pathogens 2019, 8(4), 233; https://doi.org/10.3390/pathogens8040233
Received: 25 October 2019 / Revised: 8 November 2019 / Accepted: 11 November 2019 / Published: 12 November 2019
(This article belongs to the Special Issue Animal Parasitic Diseases)
We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.
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Keywords:
Enterocytozoon hepatopenaei; qPCR; microsporidian; shrimp parasite
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style
Piamsomboon, P.; Choi, S.-K.; Hanggono, B.; Nuraini, Y.L.; Wati, F.; Tang, K.F.J.; Park, S.; Kwak, D.; Rhee, M.H.; Han, J.E.; Kim, J.H. Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR. Pathogens 2019, 8, 233.
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