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Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR

1
Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
2
Core Protein Resources Center, DGIST, Daegu 42988, Korea
3
Division of Biotechnology, DGIST, Daegu 42988, Korea
4
Fish Health and Environmental Laboratory, Brackishwater Aquaculture Development Center, Situbondo 68351, Indonesia
5
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
6
College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
7
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
*
Authors to whom correspondence should be addressed.
These authors equally contributed in this work.
Pathogens 2019, 8(4), 233; https://doi.org/10.3390/pathogens8040233
Received: 25 October 2019 / Revised: 8 November 2019 / Accepted: 11 November 2019 / Published: 12 November 2019
(This article belongs to the Special Issue Animal Parasitic Diseases)
We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms. View Full-Text
Keywords: Enterocytozoon hepatopenaei; qPCR; microsporidian; shrimp parasite Enterocytozoon hepatopenaei; qPCR; microsporidian; shrimp parasite
MDPI and ACS Style

Piamsomboon, P.; Choi, S.-K.; Hanggono, B.; Nuraini, Y.L.; Wati, F.; Tang, K.F.J.; Park, S.; Kwak, D.; Rhee, M.H.; Han, J.E.; Kim, J.H. Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR. Pathogens 2019, 8, 233.

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