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Antioxidant and Leishmanicidal Evaluation of Pulicaria Inuloides Root Extracts: A Bioguided Fractionation

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Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimique et Biologiques, Université Constantine-1, Route d’Ain El Bey, 25 000 Constantine, Algerie
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Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, Avda. Astrofısico Fco. Sanchez, S/N, 38203 La Laguna, Tenerife, Canary Islands, Spain
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Laboratoire Materiaux-Molecules et Applications, IPEST, University of Carthage, 2070 La Marsa, Tunisia
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Instituto Universitario de Bio-Orgánica Antonio González, Departamento de Química Orgánica, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Spain
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Laboratory of Biotechnology of the Bioactive Molecules and Cellular Physiopathology, Department of Biology, University of Batna, 05000 Batna, Algeria
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Authors to whom correspondence should be addressed.
Pathogens 2019, 8(4), 201; https://doi.org/10.3390/pathogens8040201
Received: 31 July 2019 / Revised: 16 October 2019 / Accepted: 19 October 2019 / Published: 23 October 2019
(This article belongs to the Special Issue Emerging Parasitic Protozoa)
Leishmaniasis remains a major world health problem, and in particular, Algeria ranks second for the incidence of cutaneous leishmaniasis. Pulicaria inuloides is a well-known Arabian Peninsula medicinal plant. In the present study, the chloroform, ethyl acetate and n-butanol extracts from the roots of Pulicaria inuloides were analyzed for antioxidant activity and its correlation with the total phenolic and flavonoid contents. The highest antioxidant activity using a DPPH assay was showed by the ethyl acetate extract (IC50 4.08 µg/mL), which also had the highest total phenolic content (307.12 µgAGE). Furthermore, P. inuloides root extracts were evaluated against Leishmania amazonensis and Leishmania donovani. The results highlighted the chloroform extract as the most active one against both tested Leishmania strains. A bioguided fractionation of the chloroform extract led to the isolation of (8R:8S)-(75:25 er)-10-isobutyryloxy-8,9-epoxy-thymol isobutyrate as the main bioactive component, showing a potent leishmanicidal activity on L. amazonensis promatigote and amastigote stages (IC50 5.03 and 2.87 µM, respectively) and a good selectivity index on murine macrophages (CC50 19.37 µM). This study provides the first report of the antioxidant and leishmanicidal activities of P. inuloides root extracts and the results point to this species as a source of potential bioactive agents. View Full-Text
Keywords: Leishmania; antioxidant; Pulicaria; bioguided; therapy Leishmania; antioxidant; Pulicaria; bioguided; therapy
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Fadel, H.; Sifaoui, I.; López-Arencibia, A.; Reyes-Batlle, M.; Jiménez, I.A.; Lorenzo-Morales, J.; Ghedadba, N.; Benayache, S.; Piñero, J.E.; Bazzocchi, I.L. Antioxidant and Leishmanicidal Evaluation of Pulicaria Inuloides Root Extracts: A Bioguided Fractionation. Pathogens 2019, 8, 201.

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