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Volume 12
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10.3390/pathogens12010079
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Article

A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis

by
César Millán-Pacheco
1,†,
Rodrigo Arreola
2,†,
Alma Villalobos-Osnaya
3,†,
Georgina Garza-Ramos
4,
Iris N. Serratos
5,
Adelaida Díaz-Vilchis
6,
Enrique Rudiño-Piñera
6 and
María Elizbeth Alvarez-Sanchez
3,*
1
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico
2
Psychiatric Genetics Department, Clinical Research Branch, National Institute of Psychiatry, Ramón de la Fuente, Calzada. México-Xochimilco 101, Colonia San Lorenzo Huipulco, Tlalpan, México City 14370, Mexico
3
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, Ciudad de Mexico 03100, Mexico
4
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Universidad # 3000, Ciudad de Mexico 04510, Mexico
5
Departamento de Química, Universidad Autónoma Metropolitana, Iztapalapa, Ciudad de Mexico 09340, Mexico
6
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca 62210, Mexico
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Pathogens 2023, 12(1), 79; https://doi.org/10.3390/pathogens12010079
Submission received: 15 November 2022 / Revised: 27 December 2022 / Accepted: 29 December 2022 / Published: 3 January 2023
(This article belongs to the Special Issue Mechanisms of Gene Expression Regulation during Host-Pathogen Interaction)
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Abstract

To understand whether protein Tv-PSP1 from Trichomonas vaginalis recognizes mRNA parasite stem-loop structures, we conducted REMSA and intrinsic fluorescence assays. We found the recombinant Tv-PSP1 structure, determined with X-ray crystallography, showed unusual thermal stability of the quaternary structure, associated with a disulfide bridge CYS76-CYS104. To gain deeper insight into the Tv-PSP1 interaction with mRNA stem-loops (mRNAsl) and its relationship with thermal stability, we also used an integrated computational protocol that combined molecular dynamics simulations, docking assays, and binding energy calculations. Docking models allowed us to determine a putative contact surface interaction region between Tv-PSP1 and mRNAsl. We determined the contributions of these complexes to the binding free energy (ΔGb) in the electrostatic (ΔGelec) and nonelectrostatic (ΔGnon-elec) components using the Adaptive Poisson–Boltzmann Solver (APBS) program. We are the first, to the best of our knowledge, to show the interaction between Tv-PSP1 and the stem-loop structures of mRNA.
Keywords: stem-loops; Trichomonas vaginalis; Tv-PSP1; REMSA; intrinsic fluorescence; molecular dynamics; molecular docking; binding free energy stem-loops; Trichomonas vaginalis; Tv-PSP1; REMSA; intrinsic fluorescence; molecular dynamics; molecular docking; binding free energy

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MDPI and ACS Style

Millán-Pacheco, C.; Arreola, R.; Villalobos-Osnaya, A.; Garza-Ramos, G.; Serratos, I.N.; Díaz-Vilchis, A.; Rudiño-Piñera, E.; Alvarez-Sanchez, M.E. A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis. Pathogens 2023, 12, 79. https://doi.org/10.3390/pathogens12010079

AMA Style

Millán-Pacheco C, Arreola R, Villalobos-Osnaya A, Garza-Ramos G, Serratos IN, Díaz-Vilchis A, Rudiño-Piñera E, Alvarez-Sanchez ME. A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis. Pathogens. 2023; 12(1):79. https://doi.org/10.3390/pathogens12010079

Chicago/Turabian Style

Millán-Pacheco, César, Rodrigo Arreola, Alma Villalobos-Osnaya, Georgina Garza-Ramos, Iris N. Serratos, Adelaida Díaz-Vilchis, Enrique Rudiño-Piñera, and María Elizbeth Alvarez-Sanchez. 2023. "A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis" Pathogens 12, no. 1: 79. https://doi.org/10.3390/pathogens12010079

APA Style

Millán-Pacheco, C., Arreola, R., Villalobos-Osnaya, A., Garza-Ramos, G., Serratos, I. N., Díaz-Vilchis, A., Rudiño-Piñera, E., & Alvarez-Sanchez, M. E. (2023). A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis. Pathogens, 12(1), 79. https://doi.org/10.3390/pathogens12010079

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