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Genotyping of Porcine Circovirus 2 (PCV-2) in Vaccinated Pigs Suffering from PCV-2-Systemic Disease between 2009 and 2020 in Spain
Article

Non-Assembled ORF2 Capsid Protein of Porcine Circovirus 2b Does Not Confer Protective Immunity

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Laboratory of Animal Welfare, Clinical Chemistry and Veterinary Immunology, Istituto Zooprofilattico Sperimentale Della Lombardia e Dell’emilia Romagna, via A. Bianchi 9, 25124 Brescia, Italy
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Diagnostic Laboratory, Istituto Zooprofilattico Sperimentale Della Lombardia e Dell’emilia Romagna, via A. Bianchi 9, 25124 Brescia, Italy
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Department of Veterinary Medical Sciences, University of Bologna, 40126 Bologna, Italy
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Genomics Department, Istituto Zooprofilattico Sperimentale Della Lombardia e Dell’emilia Romagna, via Antonio Bianchi 7/9, 25124 Brescia, Italy
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Virology Department, Istituto Zooprofilattico Sperimentale Della Lombardia e Dell’emilia Romagna, via Antonio Bianchi 7/9, 25124 Brescia, Italy
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Author to whom correspondence should be addressed.
Academic Editor: Lawrence S. Young
Pathogens 2021, 10(9), 1161; https://doi.org/10.3390/pathogens10091161
Received: 31 July 2021 / Revised: 28 August 2021 / Accepted: 7 September 2021 / Published: 9 September 2021
(This article belongs to the Special Issue Advances in Circoviruses)
Porcine Circovirus 2 (PCV2) vaccines are based on either inactivated whole virion, or recombinant ORF2 capsid protein assembled into Virus-like Particles (VLPs). No data are available about the immunizing properties of free, non-assembled capsid protein. To investigate this issue, ORF2 of a reference PCV2b strain was expressed in a Baculovirus-based expression system without assembly into VLPs. The free purified protein was formulated into an oil vaccine at three distinct Ag payloads: 10.8/3.6/1.2 micrograms/dose. Each dose was injected intramuscularly into five, 37-day old piglets, carefully matched for maternally-derived antibody. Five control piglets were injected with sterile PBS in oil adjuvant. Twenty-eight days later, all the pigs were challenged intranasally with 105.3 TCID50 of PCV2b strain DV6503. After challenge infection, all the pigs remained in good clinical conditions. The recombinant vaccine did not induce significant antibody and PCV2-specific IFN-γ responses. ELISPOT and lymphocyte proliferation data confirmed poor induction of cell-mediated immunity. In terms of PCV2 viremia, there was no significant difference between vaccinated and control animals. The histological data indicated the absence of a detectable viral load and of PCVAD lesions in both vaccinated and control animals, as well as of histiocytes and multi-nucleated giant cells. We conclude that free, non-assembled ORF2 capsid protein does not induce protective immunity. View Full-Text
Keywords: pig; Porcine Circovirus 2; ORF2 capsid protein; vaccine; protection pig; Porcine Circovirus 2; ORF2 capsid protein; vaccine; protection
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MDPI and ACS Style

Guarneri, F.; Tonni, M.; Sarli, G.; Boniotti, M.B.; Lelli, D.; Barbieri, I.; D’Annunzio, G.; Alborali, G.L.; Bacci, B.; Amadori, M. Non-Assembled ORF2 Capsid Protein of Porcine Circovirus 2b Does Not Confer Protective Immunity. Pathogens 2021, 10, 1161. https://doi.org/10.3390/pathogens10091161

AMA Style

Guarneri F, Tonni M, Sarli G, Boniotti MB, Lelli D, Barbieri I, D’Annunzio G, Alborali GL, Bacci B, Amadori M. Non-Assembled ORF2 Capsid Protein of Porcine Circovirus 2b Does Not Confer Protective Immunity. Pathogens. 2021; 10(9):1161. https://doi.org/10.3390/pathogens10091161

Chicago/Turabian Style

Guarneri, Flavia, Matteo Tonni, Giuseppe Sarli, Maria B. Boniotti, Davide Lelli, Ilaria Barbieri, Giulia D’Annunzio, Giovanni L. Alborali, Barbara Bacci, and Massimo Amadori. 2021. "Non-Assembled ORF2 Capsid Protein of Porcine Circovirus 2b Does Not Confer Protective Immunity" Pathogens 10, no. 9: 1161. https://doi.org/10.3390/pathogens10091161

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