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Article

Cystic Fibrosis Clinical Isolates of Aspergillus fumigatus Induce Similar Muco-inflammatory Responses in Primary Airway Epithelial Cells

1
Wal-Yan Respiratory Research Centre, Telethon Kids Institute, University of Western Australia, Crawley 6009, Australia
2
Faculty of Health and Medical Sciences, University of Western Australia, Crawley 6009, Australia
3
PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Murdoch 6150, Australia
4
Department of Respiratory and Sleep Medicine, Perth Children’s Hospital, Nedlands 6009, Australia
5
Occupation and Environment, School of Public Health, Curtin University, Bentley 6102, Australia
*
Author to whom correspondence should be addressed.
Co-first authors.
Co-senior authors.
§
Australian Respiratory Early Surveillance Team for Cystic Fibrosis.
Western Australian Epithelial Research Program.
Academic Editor: Joanna B. Goldberg
Pathogens 2021, 10(8), 1020; https://doi.org/10.3390/pathogens10081020
Received: 1 July 2021 / Revised: 3 August 2021 / Accepted: 11 August 2021 / Published: 13 August 2021
(This article belongs to the Special Issue Respiratory Pathogens in Cystic Fibrosis)
Aspergillus is increasingly associated with lung inflammation and mucus plugging in early cystic fibrosis (CF) disease during which conidia burden is low and strains appear to be highly diverse. It is unknown whether clinical Aspergillus strains vary in their capacity to induce epithelial inflammation and mucus production. We tested the hypothesis that individual colonising strains of Aspergillus fumigatus would induce different responses. Ten paediatric CF Aspergillus isolates were compared along with two systemically invasive clinical isolates and an ATCC reference strain. Isolates were first characterised by ITS gene sequencing and screened for antifungal susceptibility. Three clusters (A−C) of Aspergillus isolates were identified by ITS. Antifungal susceptibility was variable, particularly for itraconazole. Submerged CF and non-CF monolayers as well as differentiated primary airway epithelial cell cultures were incubated with conidia for 24 h to allow germination. None of the clinical isolates were found to significantly differ from one another in either IL-6 or IL-8 release or gene expression of secretory mucins. Clinical Aspergillus isolates appear to be largely homogenous in their mucostimulatory and immunostimulatory capacities and, therefore, only the antifungal resistance characteristics are likely to be clinically important. View Full-Text
Keywords: Aspergillus; inflammation; epithelium; cystic fibrosis; host response Aspergillus; inflammation; epithelium; cystic fibrosis; host response
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MDPI and ACS Style

McLean, S.A.; Cullen, L.; Gardam, D.J.; Schofield, C.J.; Laucirica, D.R.; Sutanto, E.N.; Ling, K.-M.; Stick, S.M.; Peacock, C.S.; Kicic, A.; Garratt, L.W.; on behalf of AREST CF; WAERP. Cystic Fibrosis Clinical Isolates of Aspergillus fumigatus Induce Similar Muco-inflammatory Responses in Primary Airway Epithelial Cells. Pathogens 2021, 10, 1020. https://doi.org/10.3390/pathogens10081020

AMA Style

McLean SA, Cullen L, Gardam DJ, Schofield CJ, Laucirica DR, Sutanto EN, Ling K-M, Stick SM, Peacock CS, Kicic A, Garratt LW, on behalf of AREST CF, WAERP. Cystic Fibrosis Clinical Isolates of Aspergillus fumigatus Induce Similar Muco-inflammatory Responses in Primary Airway Epithelial Cells. Pathogens. 2021; 10(8):1020. https://doi.org/10.3390/pathogens10081020

Chicago/Turabian Style

McLean, Samantha A., Leilani Cullen, Dianne J. Gardam, Craig J. Schofield, Daniel R. Laucirica, Erika N. Sutanto, Kak-Ming Ling, Stephen M. Stick, Christopher S. Peacock, Anthony Kicic, Luke W. Garratt, on behalf of AREST CF, and WAERP. 2021. "Cystic Fibrosis Clinical Isolates of Aspergillus fumigatus Induce Similar Muco-inflammatory Responses in Primary Airway Epithelial Cells" Pathogens 10, no. 8: 1020. https://doi.org/10.3390/pathogens10081020

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