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Insects
Volume 11
Issue 7
10.3390/insects11070449
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Communication

Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle

by
Wanwan He
1,†,
Wenbo Xu
1,†,
Kaiyun Fu
2,
Wenchao Guo
3 and
Jiang Zhang
1,*
1
State Key Laboratory of Biocatalysis of Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
2
Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, China
3
Institute of Microbial Application, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, China
*
Author to whom correspondence should be addressed.
†
These authors contribute equally to this work.
Insects 2020, 11(7), 449; https://doi.org/10.3390/insects11070449
Submission received: 11 June 2020 / Revised: 10 July 2020 / Accepted: 13 July 2020 / Published: 16 July 2020
(This article belongs to the Special Issue Insecticide Resistance: The Genetic Basis and Underlying Mechanisms in Potato Pests)
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Abstract

RNA interference (RNAi)-based technology has been proven as a novel approach for insect pest control. However, whether insects could evolve resistance to RNAi and the underlying mechanism is largely unknown. The target gene mutations were thought to be one of the potential ways to develop the resistance. Here we predicted the effective siRNA candidates that could be derived from dsRNA against the Colorado potato beetle (CPB) β-Actin gene (dsACT). By site-directed mutagenesis, we synthesized the dsRNAs with the defect in generation of effective siRNAs (and thus were supposed to have comparable low RNAi efficacy). We showed that, with mismatches to the target gene, all the dsRNA variants caused similar levels of silencing of target gene, mortality and larval growth retardation of CPB. Our results suggest that when the mismatch rate of dsACT and target β-Actin mRNA is less than 3%, the RNAi efficiency is not impaired in CPB, which might imply the low possibility of RNAi resistance evolving through the sequence mismatches between dsRNA and the target gene.
Keywords: RNA interference; double-stranded RNA; sequence mutation; resistance development; Colorado potato beetle RNA interference; double-stranded RNA; sequence mutation; resistance development; Colorado potato beetle

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MDPI and ACS Style

He, W.; Xu, W.; Fu, K.; Guo, W.; Zhang, J. Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle. Insects 2020, 11, 449. https://doi.org/10.3390/insects11070449

AMA Style

He W, Xu W, Fu K, Guo W, Zhang J. Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle. Insects. 2020; 11(7):449. https://doi.org/10.3390/insects11070449

Chicago/Turabian Style

He, Wanwan, Wenbo Xu, Kaiyun Fu, Wenchao Guo, and Jiang Zhang. 2020. "Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle" Insects 11, no. 7: 449. https://doi.org/10.3390/insects11070449

APA Style

He, W., Xu, W., Fu, K., Guo, W., & Zhang, J. (2020). Low Mismatch Rate between Double-Stranded RNA and Target mRNA Does Not Affect RNA Interference Efficiency in Colorado Potato Beetle. Insects, 11(7), 449. https://doi.org/10.3390/insects11070449

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