New Insight into the Systematics of European Lepidocyrtus (Collembola: Entomobryidae) Using Molecular and Morphological Data
Abstract
:1. Introduction
2. Materials and Methods
2.1. Species Sampling and Processing
2.2. Molecular Methods
2.2.1. DNA Extraction and Sequencing
2.2.2. Data Analyses
2.2.3. Phylogenetic Analyses
3. Results
Molecular Analyses
4. Discussion
4.1. Subgeneric Aspects of the European Lepidocyrtus
4.2. Lepidocyrtus Species Groups
4.3. Cryptic Species
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Genus | Species | Nr of Spec. | Municipality | Habitat | Position (WGS 84) | Date of Collection |
---|---|---|---|---|---|---|
Lepidocyrtus | arrabonicus | 4 | Fertőújlak (loc. typ.) | meadow | N47°40′58″ E16°50′37″ | 20.xi.2015 |
Lepidocyrtus | curvicollis | 5 | Bajót, Gerecse | cave | N47°43′22″ E18°34′31″ | 01.vi.2018 |
Lepidocyrtus | cyaneus | 4 | Tüskevár | grassland | N47°07′07″ E17°19′51″ | 03.xii.2015 |
Lepidocyrtus | florae | 4 | Hanság (loc. typ.) | alder forest | N47°40′47″ E17°07′53″ | 11.iv.2016 |
Lepidocyrtus | isabelleae | 5 | Nagykapornak | beech forest | N46°50′30″ E16°58′32″ | 15.vii.2017 |
Lepidocyrtus | isabelleae | 4 | Rum (loc. typ.) | riverine forest | N47°05′40″ E16°49′41″ | 09.viii.2016 |
Lepidocyrtus | lanuginosus | 5 | Sopron | urban park | N47°40′53″ E16°34′29″ | 02.iv.2016 |
Lepidocyrtus | lignorum | 3 | Sopron | grassland | N47°41′54″ E16°38′22″ | 24.x.2015 |
Lepidocyrtus | lignorum | 1 | Darvastó | oak forest | N46°58′56″ E17°22′12″ | 04.xii.2015 |
Lepidocyrtus | lignorum | 4 | Kerecsend | oak forest | N47°47′23″ E20°19′27″ | 01.x.2014 |
Lepidocyrtus | mariani | 2 | Nagykapornak | beech forest | N46°50′31″ E16°58′32″ | 01.vii.2014 |
Lepidocyrtus | mariani | 3 | Porva (loc. typ.) | alder forest | N47°18′42″ E17°47′30″ | 25.ix.2014 |
Lepidocyrtus | nigrescens | 3 | Kerecsend | grassland | N47°47′23″ E20°19′27″ | 01.x.2014 |
Lepidocyrtus | nigrescens | 1 | Darány | sedge | N45°58′03″ E17°32′18″ | 03.xi.2016 |
Lepidocyrtus | paradoxus | 2 | Fertőújlak | meadow | N47°40′58″ E16°50′37″ | 20.xi.2015 |
Lepidocyrtus | paradoxus | 2 | Bakonygyepes | we meadow | N47°08′29″ E17°33′37″ | 02.xii.2015 |
Lepidocyrtus | peisonis | 4 | Fertőrákos (loc. typ.) | reed-bed | N47°42′54″ E16°40′14″ | 25.iii.2017 |
Lepidocyrtus | peisonis | 3 | Darány | wet meadow | N45°59′14″ E17°33′23″ | 03.xi.2016 |
Lepidocyrtus | serbicus | 5 | Mátrafüred | beech | N47°53′26″ E19°56′37″ | 27.10.2016 |
Lepidocyrtus | tomosvaryi | 5 | Tüskevár (loc. typ.) | ash forest | N47°07′13″ E17°20′04″ | 02.xii.2015 |
Lepidocyrtus | traseri | 3 | Börzsöny (loc. typ.) | grassland | N47°53′55″ E18°51′08″ | 08.x.2014 |
Lepidocyrtus | violaceus | 5 | Csákvár | oak forest | N47°25′33″ E18°25′51″ | 13.ix.2016 |
Orchesella | cincta | 1 | Somlóvásárhely | grassland | N47° 8′43″ E17°22′35″ | 26.vi.2017 |
Cyphoderus | gr. bidenticulati | 1 | – | – | – | – |
Species Group/Species | Head Mac | Body Mac | Scales | Th. II | Abd. IV s | Abd. IV pse (dl-l) | Lateral Tuft | Dental Tubercle | Unguiculus |
---|---|---|---|---|---|---|---|---|---|
L. lanuginosus-group | |||||||||
L. cyaneus | A0 A2 A3 M2 S3 Pa5 | 10/0101+2 | – | n | – | – | – | – | A |
L. lanuginosus | A0 A2 A3 M2 S3 Pa5 | 10/0101+2 | – | n | – | – | – | – | A |
L. pallidus–serbicus group | |||||||||
L. arrabonicus | A0 A2 A3 Pa5 | 00/0101+2 | – | n | – | – | – | – | A |
L. florae | A0 A2 A3 S3 Pa5 | 00/0101+2 | – | n | – | – | – | – | A |
L. isabelleae | A0 A2 A3 M2 S3 Pa5 | 00/0101+2 | – | n | – | – | – | – | A |
L. serbicus | A0 A2 A3 M2 S3 Pa5 | 00/0101+2 | – | n | – | – | – | – | A |
L. tomosvaryi | A0 A2 A3 M2 S3 Pa5 | 00/0101+2 | – | n | – | – | – | + | A |
L. lignorum group | |||||||||
L. lignorum | A0 A2 A3 Pa5 | 00/0101+3 | + | p | – | – | – | – | A |
L. peisonis | A0 A2 A3 Pa5 | 00/0101+3 | + | p | – | – | + | – | T |
L. traseri | A0 A2 A3 Pa5 | 00/0101+3 | + | p | – | – | – | – | A |
L. violaceus | A0 A2 A3 Pa5 | 00/0101+3 | + | p | – | – | – | – | A |
L. curvicollis group | |||||||||
L. curvicollis | A0 A2 Pa5 | 00/0101+3 | + | P | + | + | – | – | A |
L. mariani | A0 A2 Pa5 | 00/0101+3 | + | P | + | + | – | – | A |
L. nigrescens | A0 A2 Pa5 | 00/0101+3 | + | P | + | – | – | – | A |
L. paradoxus | A0 A2 Pa5 | 00/0101+3 | + | P | + | – | – | – | A |
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Winkler, D.; Mateos, E.; Traser, G.; Lakatos, F.; Tóth, V. New Insight into the Systematics of European Lepidocyrtus (Collembola: Entomobryidae) Using Molecular and Morphological Data. Insects 2020, 11, 302. https://doi.org/10.3390/insects11050302
Winkler D, Mateos E, Traser G, Lakatos F, Tóth V. New Insight into the Systematics of European Lepidocyrtus (Collembola: Entomobryidae) Using Molecular and Morphological Data. Insects. 2020; 11(5):302. https://doi.org/10.3390/insects11050302
Chicago/Turabian StyleWinkler, Daniel, Eduardo Mateos, György Traser, Ferenc Lakatos, and Viktória Tóth. 2020. "New Insight into the Systematics of European Lepidocyrtus (Collembola: Entomobryidae) Using Molecular and Morphological Data" Insects 11, no. 5: 302. https://doi.org/10.3390/insects11050302