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Article

Zika Virus Envelope Protein Domain III Produced in K. phaffii Has the Potential for Diagnostic Applications

by
John Willians Oliveira Prates
1,
Mariana Fonseca Xisto
2,
Jojo Silva Rodrigues
1,
João Pedro Cruz Colombari
3,
Júlia Maria Alves Meira
3,
Roberto Sousa Dias
2,
Cynthia Canedo da Silva
1 and
e Sérgio Oliveira de Paula
2,*
1
Department of Microbiology, Federal University of Viçosa, Viçosa 36570-900, Minas Gerais, Brazil
2
Laboratory of Molecular Immunovirology, Department of General Biology, Federal University of Viçosa, Viçosa 36570-900, Minas Gerais, Brazil
3
Department of Medicine and Nursing, Federal University of Viçosa, Viçosa 36570-900, Minas Gerais, Brazil
*
Author to whom correspondence should be addressed.
Diagnostics 2022, 12(5), 1198; https://doi.org/10.3390/diagnostics12051198
Submission received: 24 March 2022 / Revised: 29 April 2022 / Accepted: 9 May 2022 / Published: 11 May 2022
(This article belongs to the Section Diagnostic Microbiology and Infectious Disease)
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Abstract

Zika virus (ZIKV) represents a global human health threat and it is related to severe diseases such as congenital Zika syndrome (CZS) and Guillain-Barré syndrome (GBS). There is no vaccine available nor specific antiviral treatment, so developing sensitive, specific, and low-cost diagnostic tests is necessary. Thus, the objective of this work was to produce the Zika virus envelope protein domain III (ZIKV-EDIII) in Komagataella phaffii KM71H and evaluate its potential for diagnostic applications. After the K. phaffii had been transformed with the pPICZαA-ZIKV-EDIII vector, an SDS-PAGE and Western Blot were performed to characterize the recombinant protein and an ELISA to evaluate the antigenic potential. The results show that ZIKV-EDIII was produced in the expected size, with a good purity grade and yield of 2.58 mg/L. The receiver operating characteristic (ROC) curve showed 90% sensitivity and 87.5% specificity for IgM, and 93.33% sensitivity and 82.76% specificity for IgG. The ZIKV-EDIII protein was efficiently produced in K. phaffi, and it has the potential for diagnostic applications.
Keywords: Zika virus; protein E; domain III; Komagataella phaffii; diagnostic Zika virus; protein E; domain III; Komagataella phaffii; diagnostic

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MDPI and ACS Style

Prates, J.W.O.; Xisto, M.F.; Rodrigues, J.S.; Colombari, J.P.C.; Meira, J.M.A.; Dias, R.S.; Silva, C.C.d.; de Paula, e.S.O. Zika Virus Envelope Protein Domain III Produced in K. phaffii Has the Potential for Diagnostic Applications. Diagnostics 2022, 12, 1198. https://doi.org/10.3390/diagnostics12051198

AMA Style

Prates JWO, Xisto MF, Rodrigues JS, Colombari JPC, Meira JMA, Dias RS, Silva CCd, de Paula eSO. Zika Virus Envelope Protein Domain III Produced in K. phaffii Has the Potential for Diagnostic Applications. Diagnostics. 2022; 12(5):1198. https://doi.org/10.3390/diagnostics12051198

Chicago/Turabian Style

Prates, John Willians Oliveira, Mariana Fonseca Xisto, Jojo Silva Rodrigues, João Pedro Cruz Colombari, Júlia Maria Alves Meira, Roberto Sousa Dias, Cynthia Canedo da Silva, and e Sérgio Oliveira de Paula. 2022. "Zika Virus Envelope Protein Domain III Produced in K. phaffii Has the Potential for Diagnostic Applications" Diagnostics 12, no. 5: 1198. https://doi.org/10.3390/diagnostics12051198

APA Style

Prates, J. W. O., Xisto, M. F., Rodrigues, J. S., Colombari, J. P. C., Meira, J. M. A., Dias, R. S., Silva, C. C. d., & de Paula, e. S. O. (2022). Zika Virus Envelope Protein Domain III Produced in K. phaffii Has the Potential for Diagnostic Applications. Diagnostics, 12(5), 1198. https://doi.org/10.3390/diagnostics12051198

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