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Open AccessArticle

Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J

1
Instituto Oftalmológico Fernández-Vega, Avenida Doctores Fernández-Vega, 34, 33012 Oviedo, Spain
2
Instituto Universitario Fernández-Vega (Fundación de Investigación Oftalmológica, Universidad de Oviedo), 33012 Oviedo, Spain
3
Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, CT 06510, USA
*
Authors to whom correspondence should be addressed.
Both authors contributed equally to this work.
Diagnostics 2020, 10(6), 425; https://doi.org/10.3390/diagnostics10060425
Received: 22 May 2020 / Revised: 18 June 2020 / Accepted: 19 June 2020 / Published: 23 June 2020
(This article belongs to the Special Issue Corneal and Retinal Diseases: Diagnostic and Therapeutic Approaches)
Animal models of glaucoma, a neurodegenerative disease affecting the retina, offer the opportunity to study candidate molecular biomarkers throughout the disease. In this work, the DBA/2J glaucomatous mouse has been used to study the systemic levels of several proteins previously identified as potential biomarkers of glaucoma, along the pre- to post-glaucomatous transition. Serum samples obtained from glaucomatous and control mice at 4, 10, and 14 months, were classified into different experimental groups according to the optic nerve damage at 14 months old. Quantifications of ten serum proteins were carried out by enzyme immunoassays. Changes in the levels of some of these proteins in the transition to glaucomatous stages were identified, highlighting the significative decrease in the concentration of complement C4a protein. Moreover, the five-protein panel consisting of complement C4a, complement factor H, ficolin-3, apolipoprotein A4, and transthyretin predicted the transition to glaucoma in 78% of cases, and to the advanced disease in 89%. Our data, although still preliminary, suggest that disease development in DBA/2J mice is associated with important molecular changes in immune response and complement system proteins and demonstrate the utility of this model in identifying, at systemic level, potential markers for the diagnosis of glaucoma. View Full-Text
Keywords: glaucoma; DBA/2J mouse model; blood; biomarkers; immune response; complement system glaucoma; DBA/2J mouse model; blood; biomarkers; immune response; complement system
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MDPI and ACS Style

Fernández-Vega Cueto, A.; Álvarez, L.; García, M.; Artime, E.; Álvarez Barrios, A.; Rodríguez-Uña, I.; Coca-Prados, M.; González-Iglesias, H. Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J. Diagnostics 2020, 10, 425.

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