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Article

Calcifying Bacteria Flexibility in Induction of CaCO3 Mineralization

by
Darya A. Golovkina
1,2,
Elena V. Zhurishkina
1,2,
Lyubov A. Ivanova
1,2,
Alexander E. Baranchikov
3,
Alexey Y. Sokolov
1,
Kirill S. Bobrov
1,2,
Alexey E. Masharsky
4,
Natalia V. Tsvigun
5,
Gennady P. Kopitsa
1 and
Anna A. Kulminskaya
1,2,*
1
Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, Russia
2
Kurchatov Genome Centre-PNPI, 188300 Gatchina, Russia
3
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, 119991 Moscow, Russia
4
Core Facility Centre for Molecular and Cell Technologies, St. Petersburg State University, 198504 St. Petersburg, Russia
5
Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 119333 Moscow, Russia
*
Author to whom correspondence should be addressed.
Life 2020, 10(12), 317; https://doi.org/10.3390/life10120317
Submission received: 29 October 2020 / Revised: 24 November 2020 / Accepted: 26 November 2020 / Published: 28 November 2020
(This article belongs to the Section Microbiology)
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Abstract

Microbially induced CaCO3 precipitation (MICP) is considered as an alternative green technology for cement self-healing and a basis for the development of new biomaterials. However, some issues about the role of bacteria in the induction of biogenic CaCO3 crystal nucleation, growth and aggregation are still debatable. Our aims were to screen for ureolytic calcifying microorganisms and analyze their MICP abilities during their growth in urea-supplemented and urea-deficient media. Nine candidates showed a high level of urease specific activity, and a sharp increase in the urea-containing medium pH resulted in efficient CaCO3 biomineralization. In the urea-deficient medium, all ureolytic bacteria also induced CaCO3 precipitation although at lower pH values. Five strains (B. licheniformis DSMZ 8782, B. cereus 4b, S. epidermidis 4a, M. luteus BS52, M. luteus 6) were found to completely repair micro-cracks in the cement samples. Detailed studies of the most promising strain B. licheniformis DSMZ 8782 revealed a slower rate of the polymorph transformation in the urea-deficient medium than in urea-containing one. We suppose that a ureolytic microorganism retains its ability to induce CaCO3 biomineralization regardless the origin of carbonate ions in a cell environment by switching between mechanisms of urea-degradation and metabolism of calcium organic salts.
Keywords: calcium carbonate; biomineralization; ureolytic bacteria; polymorph calcium carbonate; biomineralization; ureolytic bacteria; polymorph

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MDPI and ACS Style

Golovkina, D.A.; Zhurishkina, E.V.; Ivanova, L.A.; Baranchikov, A.E.; Sokolov, A.Y.; Bobrov, K.S.; Masharsky, A.E.; Tsvigun, N.V.; Kopitsa, G.P.; Kulminskaya, A.A. Calcifying Bacteria Flexibility in Induction of CaCO3 Mineralization. Life 2020, 10, 317. https://doi.org/10.3390/life10120317

AMA Style

Golovkina DA, Zhurishkina EV, Ivanova LA, Baranchikov AE, Sokolov AY, Bobrov KS, Masharsky AE, Tsvigun NV, Kopitsa GP, Kulminskaya AA. Calcifying Bacteria Flexibility in Induction of CaCO3 Mineralization. Life. 2020; 10(12):317. https://doi.org/10.3390/life10120317

Chicago/Turabian Style

Golovkina, Darya A., Elena V. Zhurishkina, Lyubov A. Ivanova, Alexander E. Baranchikov, Alexey Y. Sokolov, Kirill S. Bobrov, Alexey E. Masharsky, Natalia V. Tsvigun, Gennady P. Kopitsa, and Anna A. Kulminskaya. 2020. "Calcifying Bacteria Flexibility in Induction of CaCO3 Mineralization" Life 10, no. 12: 317. https://doi.org/10.3390/life10120317

APA Style

Golovkina, D. A., Zhurishkina, E. V., Ivanova, L. A., Baranchikov, A. E., Sokolov, A. Y., Bobrov, K. S., Masharsky, A. E., Tsvigun, N. V., Kopitsa, G. P., & Kulminskaya, A. A. (2020). Calcifying Bacteria Flexibility in Induction of CaCO3 Mineralization. Life, 10(12), 317. https://doi.org/10.3390/life10120317

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