Next Article in Journal
Bias Reduction for the Marshall-Olkin Extended Family of Distributions with Application to an Airplane’s Air Conditioning System and Precipitation Data
Previous Article in Journal
An Eigenvalues Approach for a Two-Dimensional Porous Medium Based Upon Weak, Normal and Strong Thermal Conductivities
Open AccessArticle

Venlafaxine Chiral Separation by Capillary Electrophoresis Using Cyclodextrin Derivatives as Chiral Selector and Experimental Design Method Optimization

1
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine, Pharmacy, Science and Technology “G.E. Palade” of Târgu Mureș, Gh Marinescu 32, 540142 Târgu Mureș, Romania
2
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University of Bratislava, Odbojárov 10, 83232 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Symmetry 2020, 12(5), 849; https://doi.org/10.3390/sym12050849
Received: 2 May 2020 / Revised: 18 May 2020 / Accepted: 20 May 2020 / Published: 22 May 2020
Venlafaxine (VFX) is a modern antidepressant from the serotonin and norepinephrine reuptake inhibitor (SNRI) class. It is a chiral substance used in therapy as a racemate, but differences between the pharmacological properties of the two enantiomers have been reported. The current article presents the development of a simple capillary electrophoresis (CE) method for the rapid chiral separation of VFX enantiomers. A complex cyclodextrin (CD) screening at four different pH levels was carried out to establish the optimum chiral selector; carboxymethyl-β-CD (CM-β-CD) at pH 2.5 was selected for further method development. An initial “one factor at time” (OFAT) screening strategy was used to establish the influence of analytical parameters on the separation, followed by a face centered central composite design (FCCD) for the optimization process. The analytical performances of the newly developed method were verified in terms of accuracy, linearity, precision, repeatability, and sensitivity. The method was used for the determination of VFX enantiomer ratio in pharmaceutical forms. Finally, computer modelling of VFX-CD complexes was undertaken to characterize host–guest chiral recognition. View Full-Text
Keywords: venlafaxine; chiral separation; capillary electrophoresis; cyclodextrins; experimental design venlafaxine; chiral separation; capillary electrophoresis; cyclodextrins; experimental design
Show Figures

Graphical abstract

MDPI and ACS Style

Milan, A.; Hancu, G.; Lupu, D.; Budău, M.; Garaj, V.; Kelemen, H. Venlafaxine Chiral Separation by Capillary Electrophoresis Using Cyclodextrin Derivatives as Chiral Selector and Experimental Design Method Optimization. Symmetry 2020, 12, 849.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop