Matrix Metalloproteinase Genes (MMP1, MMP10, MMP12) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures
Abstract
:1. Introduction
2. Materials and Methods
Statistical Analyses
3. Results
4. Discussion
Implications
- Variants within the MMP1, (rs1799750 - >G), MMP10 (rs486055 C > T) and MMP12 (rs2276109 T > C) genes may not modulate susceptibility to ACL injuries.
- Interactions of different gene variants in several genes that code for structural and regulatory elements of ligaments are responsible for genetic susceptibility to ACL rupture.
- The interactions of MMP1 MMP10, MMP12 genetic variants in determining risk susceptibility may not contribute to future multifactorial risk models to ACL rupture.
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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SNP | MAF (%) | ACLR + CON | ACLR | CON |
---|---|---|---|---|
MMP1 (-/G rs1799750) | allele G (47.7) | 0.562 | 0.507 | 1.0 |
MMP10 (C/T rs486055) | allele T (17.6) | 0.616 | 0.461 | 0.826 |
MMP12 (T/C rs2276109) | allele C (15.9) | 0.591 | 1.0 | 0.582 |
Polymorphism | Model | CON (n = 202) | % | ACLR # (n = 227/228) | % | OR | 95% CI | p * | |
---|---|---|---|---|---|---|---|---|---|
MMP1 rs1799750 -/G | Codominant | ||||||||
-- | 55 | 27.23 | 59 | 25.99 | 1.00 | 0.922/0.924 | |||
-G | 102 | 50.50 | 119 | 52.42 | 1.09 | 0.69 | 1.71 | ||
GG | 45 | 22.28 | 49 | 21.59 | 1.02 | 0.59 | 1.75 | ||
Dominant | |||||||||
-- | 55 | 27.23 | 59 | 25.99 | 1.00 | 0.772/0.774 | |||
-G + GG | 147 | 72.77 | 168 | 74.01 | 1.07 | 0.69 | 1.64 | ||
Recessive | |||||||||
-- + -G | 157 | 77.72 | 178 | 78.41 | 1.00 | 0.863/0.865 | |||
GG | 45 | 22.28 | 49 | 21.59 | 0.96 | 0.61 | 1.52 | ||
Overdominant | |||||||||
-- + GG | 100 | 49.50 | 108 | 47.58 | 1.00 | 0.690/0.693 | |||
-G | 102 | 50.50 | 119 | 52.42 | 1.08 | 0.74 | 1.58 | ||
MMP10 rs486055 C/T | Codominant | ||||||||
CC | 131 | 64.85 | 163 | 71.49 | 1.00 | 0.335/0.400 | |||
CT | 63 | 31.19 | 58 | 25.44 | 0.74 | 0.48 | 1.13 | ||
TT | 8 | 3.96 | 7 | 3.07 | 0.70 | 0.25 | 1.99 | ||
Dominant | |||||||||
CC | 131 | 64.85 | 163 | 71.49 | 1.00 | 0.140/0.177 | |||
CT + TT | 71 | 35.15 | 65 | 28.51 | 0.74 | 0.49 | 1.11 | ||
Recessive | |||||||||
CC + CT | 194 | 96.04 | 221 | 96.93 | 1.00 | 0.616/0.773 | |||
TT | 8 | 3.96 | 7 | 3.07 | 0.77 | 0.27 | 2.16 | ||
Overdominant | |||||||||
CC + TT | 139 | 68.81 | 170 | 74.56 | 1.00 | 0.186/0.201 | |||
CT | 63 | 31.19 | 58 | 25.44 | 0.75 | 0.49 | 1.15 | ||
MMP12 rs2276109 T/C | Codominant | ||||||||
TT | 144 | 71.29 | 158 | 69.30 | 1.00 | 0.678/0.584 | |||
CT | 55 | 27.23 | 64 | 28.07 | 1.06 | 0.69 | 0.69 | ||
CC | 3 | 1.49 | 6 | 2.63 | 1.82 | 0.45 | 0.45 | ||
Dominant | |||||||||
TT | 144 | 71.29 | 158 | 69.30 | 1.00 | 0.652/0.807 | |||
CT + CC | 58 | 28.71 | 70 | 30.70 | 1.10 | 0.73 | 0.73 | ||
Recessive | |||||||||
TT + CT | 199 | 98.51 | 222 | 97.37 | 1.00 | 0.402/0.300 | |||
CC | 3 | 1.49 | 6 | 2.63 | 1.79 | 0.44 | 0.44 | ||
Overdominant | |||||||||
TT + CC | 147 | 72.77 | 164 | 71.93 | 1.00 | 0.846/0.936 | |||
CT | 55 | 27.23 | 64 | 28.07 | 1.04 | 0.68 | 0.68 |
MMP1 x MMP10 | rs486055 | p * | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CC | CT | TT | |||||||||||||||
CON (n = 131) | ACLR (n = 162) | OR | 95% CI | CON (n = 62) | ACLR (n = 58) | OR | 95% CI | CON (n = 8) | ACLR (n = 7) | OR | 95% CI | ||||||
rs1799750 | -- | 25 | 31 | 1.00 | 24 | 22 | 0.74 | 0.34 | 1.62 | 6 | 6 | 0.81 | 0.23 | 2.81 | 0.884/ 0.886 | ||
-G | 65 | 84 | 1.04 | 0.56 | 1.93 | 35 | 34 | 0.78 | 0.39 | 1.59 | 2 | 1 | 0.40 | 0.03 | 4.71 | ||
GG | 41 | 47 | 0.92 | 0.47 | 1.81 | 4 | 2 | 0.40 | 0.07 | 2.38 | 0 | 0 | NA | NA | NA |
MMP1 x MMP12 | rs2276109 | p * | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TT | CT | CC | |||||||||||||||
CON (n = 144) | ACLR (n = 158) | OR | 95% CI | CON (n = 55) | ACLR (n = 63) | OR | 95% CI | CON (n = 3) | ACLR (n = 6) | OR | 95% CI | ||||||
rs1799750 | -- | 48 | 51 | 1.00 | NA | NA | 6 | 8 | 1.25 | 0.41 | 3.88 | 1 | 0 | 0.00 | 0.00 | NA | 0.412/ 0.347 |
-G | 71 | 84 | 1.11 | 0.67 | 1.85 | 31 | 35 | 1.06 | 0.57 | 1.98 | 0 | 0 | NA | NA | NA | ||
GG | 25 | 23 | 0.87 | 0.43 | 1.73 | 18 | 20 | 1.05 | 0.49 | 2.21 | 2 | 6 | 2.82 | 0.54 | 14.67 |
MMP10 x MMP12 | rs2276109 | p * | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TT | TC | CC | |||||||||||||||
CON (n = 144) | ACLR (n = 158) | OR | 95% CI | CON (n = 55) | ACLR (n = 64) | OR | 95% CI | CON (n = 3) | ACLR (n = 6) | OR | 95% CI | ||||||
rs486055 | CC | 91 | 111 | 1.00 | NA | NA | 38 | 47 | 1.01 | 0.61 | 1.69 | 2 | 5 | 2.05 | 0.39 | 10.81 | 0.586/ 0.553 |
CT | 46 | 43 | 0.77 | 0.46 | 1.26 | 16 | 14 | 0.72 | 0.33 | 1.55 | 1 | 1 | 0.82 | 0.05 | 13.29 | ||
TT | 7 | 4 | 0.47 | 0.13 | 1.65 | 1 | 3 | 2.46 | 0.25 | 24.05 | 0 | 0 | NA | NA | NA |
Haplotype [rs1799750, rs486055, rs2276109] | Frequency (%) | Additive (Score = 2.95, p = 0.890) | Dominant (Score = 2.40, p = 0.966) | Recessive (Score = 2.30, p = 0.680) | |||||
---|---|---|---|---|---|---|---|---|---|
ACLR + CON | ACLR | CON | Score | p * | Score | p * | Score | p * | |
[GTT] | 8.55 | 7.24 | 10.37 | -1.29 | 0.197/0.225 | -1.22 | 0.221/0.239 | NA | NA |
[-TT] | 6.87 | 6.23 | 7.61 | -0.71 | 0.480/0.613 | -0.49 | 0.626/0.648 | -0.89 | 0.372/0.441 |
[-CC] | 0.60 | 0.53 | 0.68 | -0.21 | 0.836/0.648 | -0.21 | 0.836/0.769 | NA | NA |
[-TC] | 1.27 | 1.23 | 1.30 | -0.16 | 0.870/0.795 | -0.16 | 0.870/0.795 | NA | NA |
[-CT] | 17.81 | 17.95 | 17.64 | 0.08 | 0.936/0.979 | -0.06 | 0.949/0.917 | 0.25 | 0.802/0.850 |
[GTC] | 0.87 | 1.09 | 0.28 | 0.16 | 0.870/0.811 | 0.16 | 0.870/0.887 | NA | NA |
[GCT] | 50.84 | 51.91 | 49.29 | 0.48 | 0.634/0.638 | 0.22 | 0.824/0.811 | 0.60 | 0.549/0.503 |
[GCC] | 13.20 | 13.81 | 12.84 | 0.77 | 0.443/0.487 | 0.55 | 0.580/677 | 0.98 | 0.325/0.240 |
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Lulińska, E.; Gibbon, A.; Kaczmarczyk, M.; Maciejewska-Skrendo, A.; Ficek, K.; Leońska-Duniec, A.; Wilk, M.; Leźnicka, K.; Michałowska-Sawczyn, M.; Humińska-Lisowska, K.; et al. Matrix Metalloproteinase Genes (MMP1, MMP10, MMP12) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures. Genes 2020, 11, 766. https://doi.org/10.3390/genes11070766
Lulińska E, Gibbon A, Kaczmarczyk M, Maciejewska-Skrendo A, Ficek K, Leońska-Duniec A, Wilk M, Leźnicka K, Michałowska-Sawczyn M, Humińska-Lisowska K, et al. Matrix Metalloproteinase Genes (MMP1, MMP10, MMP12) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures. Genes. 2020; 11(7):766. https://doi.org/10.3390/genes11070766
Chicago/Turabian StyleLulińska, Ewelina, Andrea Gibbon, Mariusz Kaczmarczyk, Agnieszka Maciejewska-Skrendo, Krzysztof Ficek, Agata Leońska-Duniec, Michał Wilk, Katarzyna Leźnicka, Monika Michałowska-Sawczyn, Kinga Humińska-Lisowska, and et al. 2020. "Matrix Metalloproteinase Genes (MMP1, MMP10, MMP12) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures" Genes 11, no. 7: 766. https://doi.org/10.3390/genes11070766
APA StyleLulińska, E., Gibbon, A., Kaczmarczyk, M., Maciejewska-Skrendo, A., Ficek, K., Leońska-Duniec, A., Wilk, M., Leźnicka, K., Michałowska-Sawczyn, M., Humińska-Lisowska, K., Buryta, R., Cięszczyk, P., Maculewicz, E., Czarny, W., September, A. V., & Sawczuk, M. (2020). Matrix Metalloproteinase Genes (MMP1, MMP10, MMP12) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures. Genes, 11(7), 766. https://doi.org/10.3390/genes11070766