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Article

Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation

Department of Life and Environmental Sciences, University of Cagliari, via Tommaso Fiorelli 1, 09126 Cagliari, Italy
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Genes 2020, 11(11), 1319; https://doi.org/10.3390/genes11111319
Received: 30 September 2020 / Revised: 31 October 2020 / Accepted: 3 November 2020 / Published: 6 November 2020
(This article belongs to the Special Issue Fish Cytogenetics: Present and Future)
The Muraenidae is one of the largest and most complex anguilliform families. Despite their abundance and important ecological roles, morays are little studied, especially cytogenetically, and both their phylogenetic relationships and the taxonomy of their genera are controversial. With the aim of extending the karyology of this fish group, the chromosomal mapping of the 5S ribosomal gene family was performed on seven species belonging to the genera Muraena and Gymnothorax from both the Atlantic and Pacific oceans. Fluorescence in situ hybridisation (FISH) experiments were realized using species-specific 5S rDNA probes; in addition, two-colour FISH was performed to investigate the possible association with the 45S ribosomal gene family. Multiple 5S rDNA clusters, located either in species-specific or in possibly homoeologous chromosomes, were found. Either a syntenic or different chromosomal location of the two ribosomal genes was detected. Our results revealed variability in the number and location of 5S rDNA clusters and confirmed a substantial conservation of the number and location of the 45S rDNA. View Full-Text
Keywords: comparative cytogenetics; FISH; Gymnothorax; Muraena; Muraenidae; ribosomal genes comparative cytogenetics; FISH; Gymnothorax; Muraena; Muraenidae; ribosomal genes
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MDPI and ACS Style

Coluccia, E.; Deidda, F.; Lobina, C.; Melis, R.; Porcu, C.; Agus, B.; Salvadori, S. Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation. Genes 2020, 11, 1319. https://doi.org/10.3390/genes11111319

AMA Style

Coluccia E, Deidda F, Lobina C, Melis R, Porcu C, Agus B, Salvadori S. Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation. Genes. 2020; 11(11):1319. https://doi.org/10.3390/genes11111319

Chicago/Turabian Style

Coluccia, Elisabetta, Federica Deidda, Cinzia Lobina, Riccardo Melis, Cristina Porcu, Blondine Agus, and Susanna Salvadori. 2020. "Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation" Genes 11, no. 11: 1319. https://doi.org/10.3390/genes11111319

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