Effects of Continuous Sugar Beet Cropping on Rhizospheric Microbial Communities
1
Guangdong Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangdong Province Pesticide-fertilizer Technology Research Center, Guangzhou 510316, China
2
Shihezi Academy of Agricultural Sciences, Xinjiang Uygur Autonomous Region, Shihezi 832000, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(1), 13; https://doi.org/10.3390/genes11010013
Received: 5 November 2019
/
Revised: 17 December 2019
/
Accepted: 18 December 2019
/
Published: 22 December 2019
(This article belongs to the Section Microbial Genetics and Genomics)
The continuous cropping of sugar beet can result in soil degradation and a decrease in the sugar beet yield and quality. However, the role of continuous sugar beet (Beta vulgaris L. var. saccharifera) cropping in shaping the structure and function of the rhizosphere microbial community remains poorly investigated. In this study, we comparatively investigated the impact of different numbers of years of continuous sugar beet cropping on structural and functional changes in the microbial community of the rhizosphere using high-throughput sequencing and bioinformatics analysis. We collected rhizosphere soils from fields continuously cropped for one-year (T1), five-year (T5), and thirty-year (T30) periods, as well as one bulk soil (T0), in the Xinjiang Uygur Autonomous Region. The results demonstrated that continuous sugar beet cropping resulted in a significant decline in the community diversity of soil bacterial and fungal populations from T1 to T5. With continuous change in the structure of the microbial community, the Shannon diversity and observed species were increased in T30. With an abundance of pathogenic microbes, including Acidobacteria, Alternaria, and Fusarium, that were highly enriched in T30, soil-borne diseases could be accelerated, deduced by functional predictions based on 16S rRNA genes. Continuous sugar beet cropping also led to significant declines in beneficial bacteria, including Actinobacteria, Pseudomonas spp., and Bacillus spp. In addition, we profiled and analyzed predictive metabolic characteristics (metabolism and detoxification). The abundance of phenolic acid decarboxylase involved in the phenolic acid degradation pathway was significantly lower in groups T5 and T30 than that in T0 and T1, which could result in the phenolic compounds becoming excessive in long-term continuous cropping soil. Our results provide a deeper understanding of the rhizosphere soil microbial community’s response to continuous sugar beet cropping, which is important in evaluating the sustainability of this agricultural practice.
View Full-Text
Keywords:
sugar beet; continuous cropping; rhizosphere microbial community; high-throughput sequencing
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Supplementary File 1:
ZIP-Document (ZIP, 2431 KiB)
MDPI and ACS Style
Huang, W.; Sun, D.; Fu, J.; Zhao, H.; Wang, R.; An, Y. Effects of Continuous Sugar Beet Cropping on Rhizospheric Microbial Communities. Genes 2020, 11, 13. https://doi.org/10.3390/genes11010013
AMA Style
Huang W, Sun D, Fu J, Zhao H, Wang R, An Y. Effects of Continuous Sugar Beet Cropping on Rhizospheric Microbial Communities. Genes. 2020; 11(1):13. https://doi.org/10.3390/genes11010013
Chicago/Turabian StyleHuang, Weijuan, Donglei Sun, Jiantao Fu, Huanhuan Zhao, Ronghua Wang, and Yuxing An. 2020. "Effects of Continuous Sugar Beet Cropping on Rhizospheric Microbial Communities" Genes 11, no. 1: 13. https://doi.org/10.3390/genes11010013
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
