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Volume 10
Issue 7
10.3390/genes10070516
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Article

Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato

by
Dominik Marko
1,2,
Asmaa El-shershaby
1,3,
Filomena Carriero
2,
Stephan Summerer
2,
Angelo Petrozza
2,
Rina Iannacone
2,
Enrico Schleiff
1,4,5,* and
Sotirios Fragkostefanakis
1
1
Department of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany
2
ALSIA Research Center Metapontum Agrobios S.S. Jonica 106 Km 448,2 -75100 Matera, Italy
3
Department of Molecular Biology, Genetic Engineering and Biotechnology Division, National Research Centre, 12311 Dokki, Giza, Egypt
4
Frankfurt Institute of Advanced Studies (FIAS), D-60438 Frankfurt am Main, Germany
5
Buchmann Institute for Molecular Life Sciences (BMLS), Goethe University, D-60438 Frankfurt am Main, Germany
*
Author to whom correspondence should be addressed.
Genes 2019, 10(7), 516; https://doi.org/10.3390/genes10070516
Received: 30 May 2019 / Revised: 30 June 2019 / Accepted: 4 July 2019 / Published: 7 July 2019
(This article belongs to the Special Issue TILLING and CRISPR to design the varieties of tomorrow)
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Abstract

The identification of heat stress (HS)-resilient germplasm is important to ensure food security under less favorable environmental conditions. For that, germplasm with an altered activity of factors regulating the HS response is an important genetic tool for crop improvement. Heat shock binding protein (HSBP) is one of the main negative regulators of HS response, acting as a repressor of the activity of HS transcription factors. We identified a TILLING allele of Solanum lycopersicum (tomato) HSBP1. We examined the effects of the mutation on the functionality of the protein in tomato protoplasts, and compared the thermotolerance capacity of lines carrying the wild-type and mutant alleles of HSBP1. The methionine-to-isoleucine mutation in the central heptad repeats of HSBP1 leads to a partial loss of protein function, thereby reducing the inhibitory effect on Hsf activity. Mutant seedlings show enhanced basal thermotolerance, while mature plants exhibit increased resilience in repeated HS treatments, as shown by several physiological parameters. Importantly, plants that are homozygous for the wild-type or mutant HSBP1 alleles showed no significant differences under non-stressed conditions. Altogether, these results indicate that the identified mutant HSBP1 allele can be used as a genetic tool in breeding, aiming to improve the thermotolerance of tomato varieties. View Full-Text
Keywords: Solanum lycopersicum; heat stress; thermotolerance; heat shock protein; heat stress transcription factor; phenotyping; HSBP Solanum lycopersicum; heat stress; thermotolerance; heat shock protein; heat stress transcription factor; phenotyping; HSBP
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MDPI and ACS Style

Marko, D.; El-shershaby, A.; Carriero, F.; Summerer, S.; Petrozza, A.; Iannacone, R.; Schleiff, E.; Fragkostefanakis, S. Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato. Genes 2019, 10, 516. https://doi.org/10.3390/genes10070516

AMA Style

Marko D, El-shershaby A, Carriero F, Summerer S, Petrozza A, Iannacone R, Schleiff E, Fragkostefanakis S. Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato. Genes. 2019; 10(7):516. https://doi.org/10.3390/genes10070516

Chicago/Turabian Style

Marko, Dominik, Asmaa El-shershaby, Filomena Carriero, Stephan Summerer, Angelo Petrozza, Rina Iannacone, Enrico Schleiff, and Sotirios Fragkostefanakis. 2019. "Identification and Characterization of a Thermotolerant TILLING Allele of Heat Shock Binding Protein 1 in Tomato" Genes 10, no. 7: 516. https://doi.org/10.3390/genes10070516

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