Next Article in Journal
Detection of Transgenes in Gene Delivery Model Mice by Adenoviral Vector Using ddPCR
Next Article in Special Issue
Distribution of Duffy Phenotypes among Plasmodium vivax Infections in Sudan
Previous Article in Journal
Phenotypic Effects of FGF4 Retrogenes on Intervertebral Disc Disease in Dogs
Open AccessArticle

The Diversity, Multiplicity of Infection and Population Structure of P. falciparum Parasites Circulating in Asymptomatic Carriers Living in High and Low Malaria Transmission Settings of Ghana

1
Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
2
Immunology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana
3
School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana
4
Department of Parasitology, University Cheikh Anta Diop, Dakar, Senegal
5
Department of Biological Sciences, University of North Carolina at Charlotte, NC 28223, USA
6
West Africa Center for Cell Biology of Infectious Pathogens, University of Ghana, Accra, Ghana
*
Author to whom correspondence should be addressed.
Genes 2019, 10(6), 434; https://doi.org/10.3390/genes10060434
Received: 22 April 2019 / Revised: 31 May 2019 / Accepted: 3 June 2019 / Published: 7 June 2019
(This article belongs to the Special Issue Genetics and Genomics of Malaria Parasites)
Background: Diversity in Plasmodium falciparum poses a major threat to malaria control and elimination interventions. This study utilized 12 polymorphic microsatellite (MS) markers and the Msp2 marker to examine diversity, multiplicity of infection (MOI) as well as the population structure of parasites circulating in two sites separated by about 92 km and with varying malaria transmission intensities within the Greater Accra Region of Ghana. Methods: The diversity and MOI of P. falciparum parasites in 160 non-symptomatic volunteers living in Obom (high malaria transmission intensity) and Asutsuare (low malaria transmission intensity) aged between 8 and 60 years was determined using Msp2 genotyping and microsatellite analysis. Results: The prevalence of asymptomatic P. falciparum carriers as well as the parasite density of infections was significantly higher in Obom than in Asutsuare. Samples from Asutsuare and Obom were 100% and 65% clonal, respectively, by Msp2 genotyping but decreased to 50% and 5%, respectively, when determined by MS analysis. The genetic composition of parasites from Obom and Asutsuare were highly distinct, with parasites from Obom being more diverse than those from Asutsuare. Conclusion: Plasmodium falciparum parasites circulating in Obom are genetically more diverse and distinct from those circulating in Asutsuare. The MOI in samples from both Obom and Asutsuare increased when assessed by MS analysis relative to MSP2 genotyping. The TA40 and TA87 loci are useful markers for estimating MOI in high and low parasite prevalence settings. View Full-Text
Keywords: MSP2 genotyping; microsatellites; parasite; genetic diversity; malaria; multiplicity of infection MSP2 genotyping; microsatellites; parasite; genetic diversity; malaria; multiplicity of infection
Show Figures

Figure 1

MDPI and ACS Style

Abukari, Z.; Okonu, R.; Nyarko, S.B.; Lo, A.C.; Dieng, C.C.; Salifu, S.P.; Gyan, B.A.; Lo, E.; Amoah, L.E. The Diversity, Multiplicity of Infection and Population Structure of P. falciparum Parasites Circulating in Asymptomatic Carriers Living in High and Low Malaria Transmission Settings of Ghana. Genes 2019, 10, 434.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop