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Open AccessArticle

Analysis of Soybean Somatic Embryogenesis Using Chromosome Segment Substitution Lines and Transcriptome Sequencing

College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2019, 10(11), 943; https://doi.org/10.3390/genes10110943
Received: 20 October 2019 / Revised: 5 November 2019 / Accepted: 18 November 2019 / Published: 19 November 2019
(This article belongs to the Section Plant Genetics and Genomics)
Soybean is an important cash crop that is widely used as a source of vegetable protein and edible oil. The regeneration ability of soybean directly affects the application of biotechnology. In this study, we used the exogenous hormone 2,4-D to treat immature embryos. Different levels of somatic incidence were selected from the chromosome segment substitution lines (CSSLs) constructed by SN14 and ZYD00006. Transcriptome sequencing of extreme materials was performed, and 2666 differentially expressed genes were obtained. At the same time, a difference table was generated by combining the data on CSSL rearrangement. In the extreme materials, a total of 93 differentially expressed genes were predicted and were then analyzed by cluster analysis and Gene Ontology (GO) annotation. After screening and annotating the target genes, three differentially expressed genes with hormone pathways were identified. The expression patterns of the target genes were verified by real-time quantitative PCR (qRT-PCR). Haplotype polymorphism detection and linkage disequilibrium analysis were performed on the candidate gene Glyma.09g248200. This study provided more information on the regulation network of soybean somatic embryogenesis and regeneration processes, and further identified important genes in the soybean regeneration process and provided a theoretical basis for accelerating the application of biotechnology to soybean for improving its breeding efficiency. View Full-Text
Keywords: soybean; somatic embryogenesis; CSSL; transcriptome sequencing; RT-qPCR; haplotype analysis soybean; somatic embryogenesis; CSSL; transcriptome sequencing; RT-qPCR; haplotype analysis
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Li, S.-N.; Cheng, P.; Bai, Y.-Q.; Shi, Y.; Yu, J.-Y.; Li, R.-C.; Zhou, R.-N.; Zhang, Z.-G.; Wu, X.-X.; Chen, Q.-S. Analysis of Soybean Somatic Embryogenesis Using Chromosome Segment Substitution Lines and Transcriptome Sequencing. Genes 2019, 10, 943.

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