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Comparison of Multiscale Imaging Methods for Brain Research

Institute of Biochemistry I, Jena University Hospital—Friedrich Schiller University Jena, Nonnenplan 2-4, 07743 Jena, Germany
Core Facility Imaging, Leibniz Institute on Aging – Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany
Molecular Genetics Lab, Leibniz Institute on Aging—Fritz Lipmann Institute (FLI), Beutenbergstraße 11, 07745 Jena, Germany
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX13PJ, UK
Advanced Development Light Microscopy, Carl Zeiss Microscopy GmbH, Carl-Zeiss-Promenade 10, 07745 Jena, Germany
MRC Human Immunology Unit & Wolfson Imaging Center Oxford, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX39DS, UK
Dep. Biophysical Imaging, Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, and Institute for Applied Optics and Biophysics, Faculty of Physics and Astronomy, Friedrich Schiller University Jena, Max-Wien-Platz 1, 07743 Jena, Germany
Authors to whom correspondence should be addressed.
Cells 2020, 9(6), 1377;
Received: 2 April 2020 / Revised: 25 May 2020 / Accepted: 27 May 2020 / Published: 1 June 2020
(This article belongs to the Special Issue Aging and Regeneration)
A major challenge in neuroscience is how to study structural alterations in the brain. Even small changes in synaptic composition could have severe outcomes for body functions. Many neuropathological diseases are attributable to disorganization of particular synaptic proteins. Yet, to detect and comprehensively describe and evaluate such often rather subtle deviations from the normal physiological status in a detailed and quantitative manner is very challenging. Here, we have compared side-by-side several commercially available light microscopes for their suitability in visualizing synaptic components in larger parts of the brain at low resolution, at extended resolution as well as at super-resolution. Microscopic technologies included stereo, widefield, deconvolution, confocal, and super-resolution set-ups. We also analyzed the impact of adaptive optics, a motorized objective correction collar and CUDA graphics card technology on imaging quality and acquisition speed. Our observations evaluate a basic set of techniques, which allow for multi-color brain imaging from centimeter to nanometer scales. The comparative multi-modal strategy we established can be used as a guide for researchers to select the most appropriate light microscopy method in addressing specific questions in brain research, and we also give insights into recent developments such as optical aberration corrections. View Full-Text
Keywords: advanced light microscopy; super-resolution; multi-scale imaging; tissue; brain advanced light microscopy; super-resolution; multi-scale imaging; tissue; brain
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MDPI and ACS Style

Tröger, J.; Hoischen, C.; Perner, B.; Monajembashi, S.; Barbotin, A.; Löschberger, A.; Eggeling, C.; Kessels, M.M.; Qualmann, B.; Hemmerich, P. Comparison of Multiscale Imaging Methods for Brain Research. Cells 2020, 9, 1377.

AMA Style

Tröger J, Hoischen C, Perner B, Monajembashi S, Barbotin A, Löschberger A, Eggeling C, Kessels MM, Qualmann B, Hemmerich P. Comparison of Multiscale Imaging Methods for Brain Research. Cells. 2020; 9(6):1377.

Chicago/Turabian Style

Tröger, Jessica, Christian Hoischen, Birgit Perner, Shamci Monajembashi, Aurélien Barbotin, Anna Löschberger, Christian Eggeling, Michael M. Kessels, Britta Qualmann, and Peter Hemmerich. 2020. "Comparison of Multiscale Imaging Methods for Brain Research" Cells 9, no. 6: 1377.

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