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Open AccessArticle

Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles

1
UHasselt - Hasselt University, Faculty of Medicine and Life Sciences, Biomedical Research Institute (BIOMED), Agoralaan, 3590 Diepenbeek, Belgium
2
Flemish Institute for Technological Research (VITO), Health Department, Boeretang, 2400 Mol, Belgium
*
Author to whom correspondence should be addressed.
Cells 2020, 9(2), 312; https://doi.org/10.3390/cells9020312
Received: 2 December 2019 / Revised: 23 January 2020 / Accepted: 25 January 2020 / Published: 28 January 2020
(This article belongs to the Special Issue Stem Cell Therapy in Oral and Maxillofacial Region)
Blood vessel formation or angiogenesis is a key process for successful tooth regeneration. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) possess paracrine proangiogenic properties, which are, at least partially, induced by their extracellular vesicles (EVs). However, the isolation of BM-MSCs is associated with several drawbacks, which could be overcome by MSC-like cells of the teeth, called dental pulp stromal cells (DPSCs). This study aims to compare the angiogenic content and functions of DPSC and BM-MSC EVs and conditioned medium (CM). The angiogenic protein profile of DPSC- and BM-MSC-derived EVs, CM and EV-depleted CM was screened by an antibody array and confirmed by ELISA. Functional angiogenic effects were tested in transwell migration and chicken chorioallantoic membrane assays. All secretion fractions contained several pro- and anti-angiogenic proteins and induced in vitro endothelial cell motility. This chemotactic potential was higher for (EV-depleted) CM, compared to EVs with a stronger effect for BM-MSCs. Finally, BM-MSC CM, but not DPSC CM, nor EVs, increased in ovo angiogenesis. In conclusion, we showed that DPSCs are less potent in relation to endothelial cell chemotaxis and in ovo neovascularization, compared to BM-MSCs, which emphasizes the importance of choice of cell type and secretion fraction for stem cell-based regenerative therapies in inducing angiogenesis. View Full-Text
Keywords: extracellular vesicles; bone marrow-derived mesenchymal stromal cells; dental pulp stromal cells; angiogenesis extracellular vesicles; bone marrow-derived mesenchymal stromal cells; dental pulp stromal cells; angiogenesis
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MDPI and ACS Style

Merckx, G.; Hosseinkhani, B.; Kuypers, S.; Deville, S.; Irobi, J.; Nelissen, I.; Michiels, L.; Lambrichts, I.; Bronckaers, A. Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles. Cells 2020, 9, 312. https://doi.org/10.3390/cells9020312

AMA Style

Merckx G, Hosseinkhani B, Kuypers S, Deville S, Irobi J, Nelissen I, Michiels L, Lambrichts I, Bronckaers A. Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles. Cells. 2020; 9(2):312. https://doi.org/10.3390/cells9020312

Chicago/Turabian Style

Merckx, Greet; Hosseinkhani, Baharak; Kuypers, Sören; Deville, Sarah; Irobi, Joy; Nelissen, Inge; Michiels, Luc; Lambrichts, Ivo; Bronckaers, Annelies. 2020. "Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles" Cells 9, no. 2: 312. https://doi.org/10.3390/cells9020312

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