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Article

Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

by
Armance Gelaude
1,
Sylvain Slaby
1,
Katia Cailliau
1,
Matthieu Marin
1,
Arlette Lescuyer-Rousseau
1,
Caroline Molinaro
1,
Jan Nevoral
2,3,
Veronica Kučerová-Chrpová
2,
Marketa Sedmikova
2,
Jaroslav Petr
4,
Alain Martoriati
1,*,† and
Jean-François Bodart
1,†
1
CNRS, UMR 8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, Univ. Lille, F-59000 Lille, France
2
Department of Veterinary Sciences, Food and Natural Resources, Faculty of Agrobiology, Czech University of Life Sciences in Prague, Kamycka 129, 16521 Prague-Suchdol, Czech Republic
3
Biomedical Center and Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 1655/76, 32300 Pilsen, Czech Republic
4
Research Institute of Animal Production, Pratelstvi 815, 10400 Prague 10-Uhrineves, Czech Republic
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2020, 9(1), 237; https://doi.org/10.3390/cells9010237
Submission received: 30 November 2019 / Revised: 15 January 2020 / Accepted: 16 January 2020 / Published: 17 January 2020
(This article belongs to the Special Issue Manufacturing a Female Gamete: An Oocyte Story)
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Abstract

The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.
Keywords: meiosis; hydrogen sulfide; Xenopus laevis; oocyte; cell cycle meiosis; hydrogen sulfide; Xenopus laevis; oocyte; cell cycle

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MDPI and ACS Style

Gelaude, A.; Slaby, S.; Cailliau, K.; Marin, M.; Lescuyer-Rousseau, A.; Molinaro, C.; Nevoral, J.; Kučerová-Chrpová, V.; Sedmikova, M.; Petr, J.; et al. Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes. Cells 2020, 9, 237. https://doi.org/10.3390/cells9010237

AMA Style

Gelaude A, Slaby S, Cailliau K, Marin M, Lescuyer-Rousseau A, Molinaro C, Nevoral J, Kučerová-Chrpová V, Sedmikova M, Petr J, et al. Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes. Cells. 2020; 9(1):237. https://doi.org/10.3390/cells9010237

Chicago/Turabian Style

Gelaude, Armance, Sylvain Slaby, Katia Cailliau, Matthieu Marin, Arlette Lescuyer-Rousseau, Caroline Molinaro, Jan Nevoral, Veronica Kučerová-Chrpová, Marketa Sedmikova, Jaroslav Petr, and et al. 2020. "Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes" Cells 9, no. 1: 237. https://doi.org/10.3390/cells9010237

APA Style

Gelaude, A., Slaby, S., Cailliau, K., Marin, M., Lescuyer-Rousseau, A., Molinaro, C., Nevoral, J., Kučerová-Chrpová, V., Sedmikova, M., Petr, J., Martoriati, A., & Bodart, J.-F. (2020). Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes. Cells, 9(1), 237. https://doi.org/10.3390/cells9010237

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