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Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue

1
Department of Brain and Behavioural Sciences, University of Pavia, Via Forlanini 6, 27100 Pavia, Italy
2
Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, Corso S. Martino 10, 20081 Abbiategrasso, Milan, Italy
3
Genomic and post-Genomic Center, IRCCS Mondino Foundation, Via Mondino 2, 27100 Pavia, Italy
4
Department of Civil Engineering and Architecture, University of Pavia, Via Ferrata 3, 27100 Pavia, Italy
5
Department of Orthopaedics and Traumatology, Fondazione IRCCS Policlinico San Matteo, Str. Campeggi 18, 27100 Pavia, Italy
6
Department of Biology and Biotechnology ‘L. Spallanzani’, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy
7
Laboratory of Pharmacology, Department of Health Sciences, University of Milan, Via A. di Rudinì 8, 20142 Milan, Italy
8
Pediatric Research Center Fondazione Romeo ed Enrica Invernizzi, University of Milan, via Giovanni Battista Grassi 74, 20157 Milan, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2019, 8(8), 830; https://doi.org/10.3390/cells8080830
Received: 12 June 2019 / Revised: 29 July 2019 / Accepted: 3 August 2019 / Published: 5 August 2019
(This article belongs to the Special Issue Stem Cells and Degenerative Diseases)
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Abstract

Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system. The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms. Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs. 3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model. In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells. All cells were encapsulated inside the bioink, printed and cultivated for at least seven days; they all presented good viability. We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown. View Full-Text
Keywords: disease modeling; 3D bioprinting; cell culture; neuroblastoma cell line; iPSC; neural stem cell; bioink; 3D cell culture; sodium alginate; gelatin disease modeling; 3D bioprinting; cell culture; neuroblastoma cell line; iPSC; neural stem cell; bioink; 3D cell culture; sodium alginate; gelatin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Fantini, V.; Bordoni, M.; Scocozza, F.; Conti, M.; Scarian, E.; Carelli, S.; Di Giulio, A.M.; Marconi, S.; Pansarasa, O.; Auricchio, F.; Cereda, C. Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue. Cells 2019, 8, 830.

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