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Review

Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis

Department of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, Lawrence, KS 66047, USA
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Author to whom correspondence should be addressed.
Cells 2019, 8(7), 727; https://doi.org/10.3390/cells8070727
Received: 10 May 2019 / Revised: 11 July 2019 / Accepted: 12 July 2019 / Published: 15 July 2019
(This article belongs to the Special Issue Exosomes and Extracellular Vesicles in Health and Disease)
The use of extracellular vesicles, specifically exosomes, as carriers of biomarkers in extracellular spaces has been well demonstrated. Despite their promising potential, the use of exosomes in the clinical setting is restricted due to the lack of standardization in exosome isolation and analysis methods. The purpose of this review is to not only introduce the different types of extracellular vesicles but also to summarize their differences and similarities, and discuss different methods of exosome isolation and analysis currently used. A thorough understanding of the isolation and analysis methods currently being used could lead to some standardization in the field of exosomal research, allowing the use of exosomes in the clinical setting to become a reality. View Full-Text
Keywords: exosomes; isolation; analysis; exosomal marker protein; microvesicles exosomes; isolation; analysis; exosomal marker protein; microvesicles
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MDPI and ACS Style

Doyle, L.M.; Wang, M.Z. Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis. Cells 2019, 8, 727. https://doi.org/10.3390/cells8070727

AMA Style

Doyle LM, Wang MZ. Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis. Cells. 2019; 8(7):727. https://doi.org/10.3390/cells8070727

Chicago/Turabian Style

Doyle, Laura M., and Michael Z. Wang 2019. "Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis" Cells 8, no. 7: 727. https://doi.org/10.3390/cells8070727

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