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Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers

1
Department of Pathological Physiology, Faculty of Medicine, Masaryk University/Kamenice 5, CZ-625 00 Brno, Czech Republic
2
Department of Physiology, Faculty of Medicine, Masaryk University/Kamenice 5, CZ-625 00 Brno, Czech Republic
3
Department of Chemistry and Biochemistry, Mendel University, Zemedelska 1, 613 00 Brno, Czech Republic
4
Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-612 00 Brno, Czech Republic
5
Department of Otorhinolaryngology and Head and Neck Surgery, St. Anne’s Faculty Hospital, Pekarska 53, CZ-656 91 Brno, Czech Republic
6
Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Motol, First Faculty of Medicine, Charles University, V Uvalu 84, Prague 5, 150 06, Czech Republic
7
BIOCEV, First Faculty of Medicine, Charles University, Průmyslová 595, 252 50, Vestec, Czech Republic
*
Author to whom correspondence should be addressed.
These authors contributed equally to this study.
Cells 2019, 8(5), 428; https://doi.org/10.3390/cells8050428
Received: 8 February 2019 / Revised: 26 April 2019 / Accepted: 7 May 2019 / Published: 9 May 2019
(This article belongs to the Special Issue Tumor Microenvironment: Interaction and Metabolism)
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Abstract

Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of different metabolites may serve as diagnostic or prognostic markers. Blood amino acids have been identified as promising biomarkers in different cancers before, but little is known about this field in HNSCC. Blood amino acid profiles of 140 HNSCC patients were examined using high-performance liquid chromatography. Cox proportional hazards regression model was used to assess the prognostic value of amino acid concentrations in serum. Colony forming assay was used to identify the effect of amino acids that were significant in Cox proportional hazards regression models on colony forming ability of FaDu and Detroit 562 cell lines. In the multivariable Cox regression model for overall survival (OS), palliative treatment was associated with an unfavourable prognosis while high serum levels of methionine have had a positive prognostic impact. In the relapse-free survival (RFS) multivariable model, methionine was similarly identified as a positive prognostic factor, along with tumor localization in the oropharynx. Oral cavity localization and primary radio(chemo)therapy treatment strategy have been linked to poorer RFS. 1mM serine was shown to support the forming of colonies in both tested HNSCC cell lines. Effect of methionine was exactly the opposite. View Full-Text
Keywords: head and neck cancer; blood biomarkers; prognosis; colony forming assay; metabolism; amino acids head and neck cancer; blood biomarkers; prognosis; colony forming assay; metabolism; amino acids
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Vsiansky, V.; Svobodova, M.; Gumulec, J.; Cernei, N.; Sterbova, D.; Zitka, O.; Kostrica, R.; Smilek, P.; Plzak, J.; Betka, J.; Kalfert, D.; Masarik, M.; Raudenska, M. Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers. Cells 2019, 8, 428.

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