Next Article in Journal
Protein Hydroxylation by Hypoxia-Inducible Factor (HIF) Hydroxylases: Unique or Ubiquitous?
Next Article in Special Issue
Transient Receptor Potential (TRP) Channels in Health and Disease
Previous Article in Journal
RAC1 Takes the Lead in Solid Tumors
Previous Article in Special Issue
Purification of Functional Human TRP Channels Recombinantly Produced in Yeast
Open AccessArticle

Bioavailable Menthol (Transient Receptor Potential Melastatin-8 Agonist) Induces Energy Expending Phenotype in Differentiating Adipocytes

National Agri-Food Biotechnology Institute (NABI), Knowledge City-Sector 81, SAS Nagar, Punjab 140603, India
Pharmacology Division, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh 160014, India
Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education and Research (NIPER)-Raebareli, Transit campus Lucknow, Uttar Pradesh 226301, India
Regional Centre for Biotechnology, Faridabad-Gurgaon expressway, Faridabad, Haryana 121001, India
Department of Biotechnology, Panjab University, Sector-25, Chandigarh 160014, India
Department of Endocrinology of Ageing, Medical University of Lodz, Zeligowski St, No 7/9, 90-752 Lodz, Poland
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2019, 8(5), 383;
Received: 31 December 2018 / Revised: 12 March 2019 / Accepted: 19 March 2019 / Published: 26 April 2019
(This article belongs to the Special Issue TRP Channels in Health and Disease)
Recent evidence supports the role of menthol, a TRPM8 agonist, in enhanced energy expenditure, thermogenesis and BAT-like activity in classical WAT depots in a TRPM8 dependent and independent manner. The present study was designed to analyse whether oral and topical administration of menthol is bioavailable at subcutaneous adipose tissue and is sufficient to directlyinduce desired energy expenditure effects. GC-FID was performed to study menthol bioavailability in serum and subcutaneous white adipose tissue following oral and topical administration. Further, 3T3L1 adipocytes were treated with bioavailable menthol doses and different parameters (lipid accumulation, “browning/brite” and energy expenditure gene expression, metal analysis, mitochondrial complex’s gene expression) were studied. No difference was observed in serum levels but significant difference was seen in the menthol concentration on subcutaneous adipose tissues after oral and topical application. Menthol administration at bioavailable doses significantly increased “browning/brite” and energy expenditure phenotype, enhanced mitochondrial activity related gene expression, increased metal concentration during adipogenesis but did not alter the lipid accumulation as well as acute experiments were performed with lower dose of menthol on mature adipocytes In conclusion, the present study provides evidence that bioavailable menthol after single oral and topical administration is sufficient to induce “brite” phenotype in subcutaneous adipose tissue However, critical dose characterization for its clinical utility is required. View Full-Text
Keywords: adipose tissue; bioavailable; menthol; topical; TRPM8 adipose tissue; bioavailable; menthol; topical; TRPM8
Show Figures

Figure 1

MDPI and ACS Style

Khare, P.; Chauhan, A.; Kumar, V.; Kaur, J.; Mahajan, N.; Kumar, V.; Gesing, A.; Chopra, K.; Kondepudi, K.K.; Bishnoi, M. Bioavailable Menthol (Transient Receptor Potential Melastatin-8 Agonist) Induces Energy Expending Phenotype in Differentiating Adipocytes. Cells 2019, 8, 383.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop