IL-33 and MRGPRX2-Triggered Activation of Human Skin Mast Cells—Elimination of Receptor Expression on Chronic Exposure, but Reinforced Degranulation on Acute Priming
Abstract
1. Introduction
2. Materials and Methods
2.1. Isolation and Culture of Human Skin MCs
2.2. MC Treatment
2.3. Accell®-Mediated RNA Interference
2.4. Flow Cytometry
2.5. Determination of Histamine Release
2.6. β-Hexosaminidase Release Assay
2.7. Reverse Transcription-Quantitative PCR (RT-qPCR)
2.8. Immunoblot Analysis
2.9. Statistics
3. Results
3.1. Skin MCs Lose Responsiveness to MRGPRX2 Ligands and Massively Downregulate MRGPRX2 Expression after Long-Term Exposure to IL-33
3.2. IL-33-Triggered Downregulation of MRGPRX2 Is Partially JNK-Dependent, although JNK Is A Positive Regulator of MRGPRX2 in the Absence of IL-33
3.3. Short-Term Priming by IL-33 Fosters Skin MC Degranulation
3.4. Reinforced Degranulation by IL-33 Depends on p38
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, Z.; Guhl, S.; Franke, K.; Artuc, M.; Zuberbier, T.; Babina, M. IL-33 and MRGPRX2-Triggered Activation of Human Skin Mast Cells—Elimination of Receptor Expression on Chronic Exposure, but Reinforced Degranulation on Acute Priming. Cells 2019, 8, 341. https://doi.org/10.3390/cells8040341
Wang Z, Guhl S, Franke K, Artuc M, Zuberbier T, Babina M. IL-33 and MRGPRX2-Triggered Activation of Human Skin Mast Cells—Elimination of Receptor Expression on Chronic Exposure, but Reinforced Degranulation on Acute Priming. Cells. 2019; 8(4):341. https://doi.org/10.3390/cells8040341
Chicago/Turabian StyleWang, Zhao, Sven Guhl, Kristin Franke, Metin Artuc, Torsten Zuberbier, and Magda Babina. 2019. "IL-33 and MRGPRX2-Triggered Activation of Human Skin Mast Cells—Elimination of Receptor Expression on Chronic Exposure, but Reinforced Degranulation on Acute Priming" Cells 8, no. 4: 341. https://doi.org/10.3390/cells8040341
APA StyleWang, Z., Guhl, S., Franke, K., Artuc, M., Zuberbier, T., & Babina, M. (2019). IL-33 and MRGPRX2-Triggered Activation of Human Skin Mast Cells—Elimination of Receptor Expression on Chronic Exposure, but Reinforced Degranulation on Acute Priming. Cells, 8(4), 341. https://doi.org/10.3390/cells8040341