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Open AccessArticle

Invertebrate Retinal Progenitors as Regenerative Models in a Microfluidic System

1
Department of Biomedical Engineering, City College of New York, New York, NY 10031, USA
2
Department of Biomedical Engineering, The State University of New York at Binghamton, NY 13902, USA
3
Department of Biology, City College of New York, New York, NY 10031, USA
4
Department of Biomedical Engineering, Rutgers University, The State University of New Jersey, New Brunswick, NJ 08854, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Yunqing (Kevin) Kang
Cells 2019, 8(10), 1301; https://doi.org/10.3390/cells8101301
Received: 16 September 2019 / Revised: 15 October 2019 / Accepted: 16 October 2019 / Published: 22 October 2019
(This article belongs to the Special Issue Cell Biological Techniques and Cell-Biomaterial Interactions)
Regenerative retinal therapies have introduced progenitor cells to replace dysfunctional or injured neurons and regain visual function. While contemporary cell replacement therapies have delivered retinal progenitor cells (RPCs) within customized biomaterials to promote viability and enable transplantation, outcomes have been severely limited by the misdirected and/or insufficient migration of transplanted cells. RPCs must achieve appropriate spatial and functional positioning in host retina, collectively, to restore vision, whereas movement of clustered cells differs substantially from the single cell migration studied in classical chemotaxis models. Defining how RPCs interact with each other, neighboring cell types and surrounding extracellular matrixes are critical to our understanding of retinogenesis and the development of effective, cell-based approaches to retinal replacement. The current article describes a new bio-engineering approach to investigate the migratory responses of innate collections of RPCs upon extracellular substrates by combining microfluidics with the well-established invertebrate model of Drosophila melanogaster. Experiments utilized microfluidics to investigate how the composition, size, and adhesion of RPC clusters on defined extracellular substrates affected migration to exogenous chemotactic signaling. Results demonstrated that retinal cluster size and composition influenced RPC clustering upon extracellular substrates of concanavalin (Con-A), Laminin (LM), and poly-L-lysine (PLL), and that RPC cluster size greatly altered collective migratory responses to signaling from Fibroblast Growth Factor (FGF), a primary chemotactic agent in Drosophila. These results highlight the significance of examining collective cell-biomaterial interactions on bio-substrates of emerging biomaterials to aid directional migration of transplanted cells. Our approach further introduces the benefits of pairing genetically controlled models with experimentally controlled microenvironments to advance cell replacement therapies. View Full-Text
Keywords: Drosophila; collective migration; neurons; glia; fibroblast growth factor Drosophila; collective migration; neurons; glia; fibroblast growth factor
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MDPI and ACS Style

Pena, C.D.; Zhang, S.; Majeska, R.; Venkatesh, T.; Vazquez, M. Invertebrate Retinal Progenitors as Regenerative Models in a Microfluidic System. Cells 2019, 8, 1301. https://doi.org/10.3390/cells8101301

AMA Style

Pena CD, Zhang S, Majeska R, Venkatesh T, Vazquez M. Invertebrate Retinal Progenitors as Regenerative Models in a Microfluidic System. Cells. 2019; 8(10):1301. https://doi.org/10.3390/cells8101301

Chicago/Turabian Style

Pena, Caroline D.; Zhang, Stephanie; Majeska, Robert; Venkatesh, Tadmiri; Vazquez, Maribel. 2019. "Invertebrate Retinal Progenitors as Regenerative Models in a Microfluidic System" Cells 8, no. 10: 1301. https://doi.org/10.3390/cells8101301

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