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2D- and 3D-Based Intestinal Stem Cell Cultures for Personalized Medicine

The State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
Author to whom correspondence should be addressed.
Cells 2018, 7(12), 225;
Received: 4 October 2018 / Revised: 5 November 2018 / Accepted: 16 November 2018 / Published: 22 November 2018
(This article belongs to the Special Issue Stem Cells in Personalized Medicine)
PDF [1249 KB, uploaded 22 November 2018]


Colorectal cancer (CRC) is one of the most common cancers that have high occurrence and death in both males and females. As various factors have been found to contribute to CRC development, personalized therapies are critical for efficient treatment. To achieve this purpose, the establishment of patient-derived tumor models is critical for diagnosis and drug test. The establishment of three-dimensional (3D) organoid cultures and two-dimensional (2D) monolayer cultures of patient-derived epithelial tissues is a breakthrough for expanding living materials for later use. This review provides an overview of the different types of 2D- and 3D-based intestinal stem cell cultures, their potential benefits, and the drawbacks in personalized medicine in treatment of the intestinal disorders. View Full-Text
Keywords: intestinal stem cells; organoids; monolayer; cell culture; personalized medicine intestinal stem cells; organoids; monolayer; cell culture; personalized medicine

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Liu, Y.; Chen, Y.-G. 2D- and 3D-Based Intestinal Stem Cell Cultures for Personalized Medicine. Cells 2018, 7, 225.

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