CELTPLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO

Schimanski, Tom; Prantl, Lukas; Eigenberger, Andreas; Felthaus, Oliver; Loucas, Rafael; Utpatel, Kirsten; Steer, Kerstin

doi:10.3390/cells14161270

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Open AccessArticle

CELT^PLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO

by

Tom Schimanski

^1,*,†,

Lukas Prantl

^1,*,†

,

Andreas Eigenberger

^1,2

,

Oliver Felthaus

¹

,

Rafael Loucas

¹,

Kirsten Utpatel

³ and

Kerstin Steer

¹

Department of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany

²

Medical Device Lab, Regensburg Center of Biomedical Engineering (RCBE), Faculty of Mechanical Engineering, Ostbayerische Technische Hochschule Regensburg, Galgenbergstraße30, 93053 Regensburg, Germany

³

Institute of Pathology, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Cells 2025, 14(16), 1270; https://doi.org/10.3390/cells14161270 (registering DOI)

Submission received: 24 July 2025 / Revised: 5 August 2025 / Accepted: 16 August 2025 / Published: 17 August 2025

(This article belongs to the Special Issue Adipose-Derived Stem Cells for Tissue Regeneration)

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Abstract

Lipofilling has far more applications than cosmetic surgery alone. Due to its high content of stromal vascular fraction (SVF) cells, lipoaspirate can also be used to treat wounds, as its cellular components may accelerate wound healing. Using our CELT^PLUS protocol, we can increase the number of SVF cells per volume. Unfortunately, some patients require more than one treatment to achieve an optimal outcome, but would unnecessarily suffer from repeated liposuction. Therefore, our objective was to test whether cryopreserving CELT^PLUS fat could offer a solution, potentially avoiding the need for repeated liposuction procedures. DMSO was used as a cryoprotective agent for proof-of-principle testing, although other non-toxic cryoprotective agents should be considered in the future. The rest of our freezing protocol is a clinically friendly attempt to facilitate the translation into clinical practice. We tested the cryopreserved tissue using histological evaluation, metabolism measurement, SVF cell yield estimation, PCRs from both whole tissue and from cultured SVF cells, and Oil Red “O” staining. We found that freezing CELT^PLUS fat with DMSO yields better results than without cryoprotection in all evaluated methods. Until non-toxic cryoprotective agents are tested on CELT^PLUS fat, we do not recommend initiating animal or human testing.

Keywords: adipose tissue; stromal vascular fraction; extracellular matrix; cryopreservation; DMSO; CELT; CELT^PLUS

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MDPI and ACS Style

Schimanski, T.; Prantl, L.; Eigenberger, A.; Felthaus, O.; Loucas, R.; Utpatel, K.; Steer, K. CELT^PLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO. Cells 2025, 14, 1270. https://doi.org/10.3390/cells14161270

AMA Style

Schimanski T, Prantl L, Eigenberger A, Felthaus O, Loucas R, Utpatel K, Steer K. CELT^PLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO. Cells. 2025; 14(16):1270. https://doi.org/10.3390/cells14161270

Chicago/Turabian Style

Schimanski, Tom, Lukas Prantl, Andreas Eigenberger, Oliver Felthaus, Rafael Loucas, Kirsten Utpatel, and Kerstin Steer. 2025. "CELT^PLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO" Cells 14, no. 16: 1270. https://doi.org/10.3390/cells14161270

APA Style

Schimanski, T., Prantl, L., Eigenberger, A., Felthaus, O., Loucas, R., Utpatel, K., & Steer, K. (2025). CELT^PLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO. Cells, 14(16), 1270. https://doi.org/10.3390/cells14161270

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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CELT^PLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO

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