SGK1 Target Genes Involved in Heart and Blood Vessel Functions in PC12 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Line and Reagents
2.2. Real-Time PCR Assay
2.3. SDS-PAGE and Western Blotting
2.4. RNA-Seq and DEG Identification
2.5. Gene Enrichment Analysis
2.6. GEPIA2 Correlation Analysis
2.7. Statistical Analysis
3. Results
3.1. SGK1 Inhibitor GSK650394 Inhibits SGK1 Expression in PC12 Cells
3.2. RNA-Seq and DEG Analysis of GSK650394-Treated PC12 Cells
3.3. Gene Enrichment Analysis of DEGs in GSK650394-Treated PC12 Cells
3.4. Validation of Selected DEGs Related to Heart and Blood Vessel Functions
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Symbol | Description | Forward Primer Sequences (5′ > 3′) | Reverse Primer Sequences (5′ > 3′) |
---|---|---|---|
Sgk1 | serum/glucocorticoid regulated kinase 1 | TTCTGTGGCACGCCTGAGTA | GGTCGTACGGCTGCTTATGG |
Gapdh | glyceraldehyde-3-phosphate dehydrogenase | GGCAAGTTCAACGGCACAGT | ATGGGTTTCCCGTTGATGAC |
Adamts1 | ADAM metallopeptidase with thrombospondin type 1 motif, 1 | CAGGGCGCGGGAAAG | GGACACAAATCGCTTCTTCCTT |
Anpep | alanyl aminopeptidase, membrane | CCAAGGGTTTCTACATTTCCAAGA | CGGCTGCCACACCTAACAG |
Atp1a1 | ATPase Na+/K+ transporting subunit alpha 1 | CATCTTCCTCATTGGTATCATCGT | TGACGGTGGCCAGCAAA |
Atp2b4 | ATPase plasma membrane Ca2+ transporting 4 | GGGCTGCAGAGTCGCATT | TGTATAAGCTGACCATTCCGGATA |
Bmp2 | bone morphogenetic protein 2 | ACGACGTCCTCAGCGAGTTT | GGTCTCTGCTTCAGGCCAAA |
Ccl2 | C-C motif chemokine ligand 2 | CACGCTTCTGGGCCTGTT | TGAGACAGCACGTGGATGCT |
Col2a1 | collagen type II alpha 1 chain | GACTGGCGAGTCTTGCGTCTA | CTTGCTGCTCCACCAGTTCTT |
Dll1 | delta-like canonical Notch ligand 1 | CTGCACTGACCCCATTTGTCT | CCCGGTTTGTCACAATATCCA |
Dll4 | delta-like canonical Notch ligand 4 | GAATAGCGGCAGTGGTCGTAA | GGTTCCCGGCCAGGTAAA |
Errfi1 | ERBB receptor feedback inhibitor 1 | CGTGCCTTCCGATTTCAAA | CGCACCCACGGAAGCTT |
Fgfrl1 | fibroblast growth factor receptor-like 1 | CCCACCACCGTTGACCAT | TCCAGCCACTGTGGATCGT |
Foxo1 | forkhead box O1 | CCTCGAACCAGCTCAAACG | CTGCTCTGTCATGATGGGAGAA |
Fzd7 | frizzled class receptor 7 | GTGCACGGATATTGCCTACAAC | CCCGCGTCCTCTTGGTT |
Gja1 | gap junction protein alpha 1 | GGCCTTCCTGCTCATCCA | AGGTGTAGACCGCGCTCAA |
Gper1 | G protein-coupled estrogen receptor 1 | CCTGGACGAGCAGTACTACGATATC | TGATCTGCAGGAAGAGGGACAT |
Gucy1a1 | guanylate cyclase 1 soluble subunit alpha 1 | TGCTTCTCCCCGGTATCATT | TCCACGTGGCTTTCGTACAG |
Hmox1 | heme oxygenase 1 | TGATGGCCTCCTTGTACCATATC | GGTTCTGCTTGTTTCGCTCTATC |
Hrh3 | histamine receptor H3 | TCGCCATCTCCGACTTCCT | GTCAGCACATAGGGTACGTACAATG |
Id2 | inhibitor of DNA binding 2 | CGGTGAGGTCCGTTAGGAAA | CGGGAGATGCCCAAGCT |
Id3 | inhibitor of DNA binding 3 | TTAGCCTCTTGGACGACATGAA | GGACTCCCGGCACCAGTT |
Mmp2 | matrix metallopeptidase 2 | ACGCTTTTCTCGAATCCATGA | ATGCTCCCATCGACCAAAGT |
Mmp9 | matrix metallopeptidase 9 | TTCGAAGGCGACCTCAAGTG | TTCGGTGTAGCTTTGGATCCA |
Ncald | neurocalcin delta | CATAGCCCTGAGTGTGACATCAA | ACATGCTGAAGGCCCACTTC |
Nisch | Nischarin | CCCTGGAAGGCGTACACACT | AAGTTGCCTGCCAGGTTTAGG |
Npy1r | neuropeptide Y receptor Y1 | TGCTGGTCGCAGTCATGTGT | CCCAGTGGTCCATCAGTGTGT |
Nr2f2 | nuclear receptor subfamily 2 group F member 2 | CAGCACCACCGCAACCA | CCCACTTTGAGGCACTTTTTG |
Pik3r1 | phosphoinositide-3-kinase regulatory subunit 1 | GCAAGAGCCCTCTCTGAAATTTT | AGAGCTGGCTGCTGGGAAT |
Ppara | peroxisome proliferator activated receptor alpha | GCTAAAGCTGGCGTACGACAA | TGTTCCGGTTCTTTTTCTGAATC |
Sema3c | semaphorin 3C | CATTTGCGTCCAAGGATCATC | CGAAGCTCATCGAATGTTAAATACA |
Slit2 | slit guidance ligand 2 | TTCCGTGGTGCAGTTGACAT | CCATCTTCAATGCAGCTGATCT |
Smad3 | SMAD family member 3 | GGGCCTGCTGTCCAATGTT | AATGTGCCGCCTTGTAAGCT |
Smo | smoothened, frizzled class receptor | ACGAGGGTGGCCTGACTTT | GGACAGCCTTCAGGGAAGTG |
Smtn | smoothelin | AACCCCTCTTGCAGGATCATC | CGCACTCGGTCTCTCACAGA |
Sod2 | superoxide dismutase 2 | GGGCTGGCTTGGCTTCA | AGCAGGCGGCAATCTGTAA |
Tbr1 | T-box brain transcription factor 1 | TGCAAGGAAACCGGGTCTAT | CATCCAGTGAGCCCCAGTGT |
Tbx2 | T-box transcription factor 2 | ATCGTGCGAGCCAACGA | GGAAGACATAGGTGCGAAAGGT |
Tbx3 | T-box transcription factor 3 | AAGGAGACCGGAACTTCTGATG | CCTGGGCAAAGCAGTTGAA |
tbxa2r | thromboxane A2 receptor | GCCACATGGAACCAGATCTTG | TGCAGGCGTCGAAGCA |
Tnf | tumor necrosis factor | TGATCGGTCCCAACAAGGA | GGGCCATGGAACTGATGAGA |
Trpc3 | transient receptor potential cation channel subfamily C member 3 | CCTTGGGCCTTCCATTCCT | CCCCAGCCTGCTACAAGGT |
Vegfc | vascular endothelial growth factor C | GGCCCCAAACCAGTCACA | ACATGCACCGGCAGGAA |
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Li, Y.-H.; Sun, C.-C.; Chen, P.-M.; Chen, H.-H. SGK1 Target Genes Involved in Heart and Blood Vessel Functions in PC12 Cells. Cells 2023, 12, 1641. https://doi.org/10.3390/cells12121641
Li Y-H, Sun C-C, Chen P-M, Chen H-H. SGK1 Target Genes Involved in Heart and Blood Vessel Functions in PC12 Cells. Cells. 2023; 12(12):1641. https://doi.org/10.3390/cells12121641
Chicago/Turabian StyleLi, Yu-He, Chia-Cheng Sun, Po-Ming Chen, and Hsin-Hung Chen. 2023. "SGK1 Target Genes Involved in Heart and Blood Vessel Functions in PC12 Cells" Cells 12, no. 12: 1641. https://doi.org/10.3390/cells12121641