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Article

Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism

1
Department of Experimental Biology, Faculty of Biology and Animal Science, University of Environmental and Life Sciences, Norwida 27B St., 50-375 Wroclaw, Poland
2
Laboratory of Gene Expression, Institute of Biotechnology CAS, Biocev, 25250 Vestec, Czech Republic
3
Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Colins, CO 80523-1693, USA
4
International Institute of Translational Medicine, Jesionowa 11 St., 55-124 Malin, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Christian Neri
Cells 2022, 11(9), 1437; https://doi.org/10.3390/cells11091437
Received: 15 February 2022 / Revised: 4 April 2022 / Accepted: 20 April 2022 / Published: 24 April 2022
(This article belongs to the Special Issue Cellular and Clinical Mechanisms of Obesity and Its Complications)
The study aimed to investigate the influence of obesity on cellular features of equine endometrial progenitor cells (Eca EPCs), including viability, proliferation capacity, mitochondrial metabolism, and oxidative homeostasis. Eca EPCs derived from non-obese (non-OB) and obese (OB) mares were characterized by cellular phenotype and multipotency. Obesity-induced changes in the activity of Eca EPCs include the decline of their proliferative activity, clonogenic potential, mitochondrial metabolism, and enhanced oxidative stress. Eca EPCs isolated from obese mares were characterized by an increased occurrence of early apoptosis, loss of mitochondrial dynamics, and senescence-associated phenotype. Attenuated metabolism of Eca EPCs OB was related to increased expression of pro-apoptotic markers (CASP9, BAX, P53, P21), enhanced expression of OPN, PI3K, and AKT, simultaneously with decreased signaling stabilizing cellular homeostasis (including mitofusin, SIRT1, FOXP3). Obesity alters functional features and the self-renewal potential of endometrial progenitor cells. The impaired cytophysiology of progenitor cells from obese endometrium predicts lower regenerative capacity if used as autologous transplants. View Full-Text
Keywords: obesity; endometrial progenitor cells; cellular metabolism; self-renewal potential obesity; endometrial progenitor cells; cellular metabolism; self-renewal potential
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MDPI and ACS Style

Smieszek, A.; Marcinkowska, K.; Pielok, A.; Sikora, M.; Valihrach, L.; Carnevale, E.; Marycz, K. Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism. Cells 2022, 11, 1437. https://doi.org/10.3390/cells11091437

AMA Style

Smieszek A, Marcinkowska K, Pielok A, Sikora M, Valihrach L, Carnevale E, Marycz K. Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism. Cells. 2022; 11(9):1437. https://doi.org/10.3390/cells11091437

Chicago/Turabian Style

Smieszek, Agnieszka, Klaudia Marcinkowska, Ariadna Pielok, Mateusz Sikora, Lukas Valihrach, Elaine Carnevale, and Krzysztof Marycz. 2022. "Obesity Affects the Proliferative Potential of Equine Endometrial Progenitor Cells and Modulates Their Molecular Phenotype Associated with Mitochondrial Metabolism" Cells 11, no. 9: 1437. https://doi.org/10.3390/cells11091437

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