The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development

Feng, Lei; Gao, Yong-Yu; Sun, Mingwei; Li, Zi-Bin; Zhang, Qiang; Yang, Jie; Qiao, Cui; Jin, Hang; Feng, Hong-Sheng; Xian, Yu-Han; Qi, Jianxun; Gao, George F.; Liu, William J.; Gao, Feng-Shan

doi:10.3390/cells11244017

Open AccessArticle

The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development

by

Lei Feng

^1,2,†,

Yong-Yu Gao

^1,3,†,

Mingwei Sun

^2,†,

Zi-Bin Li

^1,4,

Qiang Zhang

⁵,

Jie Yang

^1,6,

Cui Qiao

^1,2,

Hang Jin

¹,

Hong-Sheng Feng

¹,

Yu-Han Xian

¹,

Jianxun Qi

²

,

George F. Gao

^2,6,*,

William J. Liu

^6,* and

Feng-Shan Gao

^1,*

¹

Department of Bioengineering, College of Life and Health, Dalian University, Dalian 116622, China

²

CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100101, China

³

College of Animal Medicine, Jilin Agricultural University, Changchun 130118, China

⁴

Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China

⁵

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

⁶

NHC Key Laboratory of Biosafety, Research Unit of Adaptive Evolution and Control of Emerging Viruses, Chinese Academy of Medical Sciences, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Cells 2022, 11(24), 4017; https://doi.org/10.3390/cells11244017

Submission received: 31 August 2022 / Revised: 6 December 2022 / Accepted: 7 December 2022 / Published: 12 December 2022

(This article belongs to the Special Issue Major Histocompatibility Complex (MHC) in Health and Disease 2022)

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Abstract

Foot-and-mouth disease virus (FMDV) poses a significant threat to the livestock industry. Through their recognition of the conserved epitopes presented by the swine leukocyte antigen (SLA), T cells play a pivotal role in the antiviral immunity of pigs. Herein, based on the peptide binding motif of SLA-2*HB01, from an original SLA-2 allele, a series of functional T-cell epitopes derived from the dominant antigen VP1 of FMDV with high binding capacity to SLA-2 were identified. Two parallel peptides, Hu64 and As64, from the O and Asia I serotypes, respectively, were both crystallized with SLA-2*HB01. Compared to SLA-1 and SLA-3, the SLA-2 structures showed the flexibility of residues in the P4, P6, and P8 positions and in their potential interface with TCR. Notably, the peptides Hu64 and As64 adopted quite similar overall conformation when bound to SLA-2*HB01. Hu64 has two different conformations, a more stable ‘chair’ conformation and an unstable ‘boat’ conformation observed in the two molecules of one asymmetric unit, whereas only a single ‘chair’ conformation was observed for As64. Both Hu64 and As64 could induce similar dominant T-cell activities. Our interdisciplinary study establishes a basis for the in-depth interpretation of the peptide presentation of SLA-I, which can be used toward the development of universal vaccines.

Keywords: SLA-2; foot-and-mouth disease virus; crystal; peptide; epitope; CTL; universal vaccine candidate

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MDPI and ACS Style

Feng, L.; Gao, Y.-Y.; Sun, M.; Li, Z.-B.; Zhang, Q.; Yang, J.; Qiao, C.; Jin, H.; Feng, H.-S.; Xian, Y.-H.; et al. The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development. Cells 2022, 11, 4017. https://doi.org/10.3390/cells11244017

AMA Style

Feng L, Gao Y-Y, Sun M, Li Z-B, Zhang Q, Yang J, Qiao C, Jin H, Feng H-S, Xian Y-H, et al. The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development. Cells. 2022; 11(24):4017. https://doi.org/10.3390/cells11244017

Chicago/Turabian Style

Feng, Lei, Yong-Yu Gao, Mingwei Sun, Zi-Bin Li, Qiang Zhang, Jie Yang, Cui Qiao, Hang Jin, Hong-Sheng Feng, Yu-Han Xian, and et al. 2022. "The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development" Cells 11, no. 24: 4017. https://doi.org/10.3390/cells11244017

APA Style

Feng, L., Gao, Y.-Y., Sun, M., Li, Z.-B., Zhang, Q., Yang, J., Qiao, C., Jin, H., Feng, H.-S., Xian, Y.-H., Qi, J., Gao, G. F., Liu, W. J., & Gao, F.-S. (2022). The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development. Cells, 11(24), 4017. https://doi.org/10.3390/cells11244017

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The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development

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