Next Article in Journal
Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes
Next Article in Special Issue
Ribosome-Induced Cellular Multipotency, an Emerging Avenue in Cell Fate Reversal
Previous Article in Journal
YTHDF1 Promotes Cyclin B1 Translation through m6A Modulation and Contributes to the Poor Prognosis of Lung Adenocarcinoma with KRAS/TP53 Co-Mutation
Previous Article in Special Issue
Regulation of Adult Mammalian Neural Stem Cells and Neurogenesis by Cell Extrinsic and Intrinsic Factors
Article

Myogenic Differentiation of iPS Cells Shows Different Efficiency in Simultaneous Comparison of Protocols

1
Department of Transplantation, Medical College, Jagiellonian University, 31-008 Krakow, Poland
2
Department of Transplantation, Institute of Pediatrics, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Krakow, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Christian Dani
Cells 2021, 10(7), 1671; https://doi.org/10.3390/cells10071671
Received: 25 May 2021 / Revised: 17 June 2021 / Accepted: 28 June 2021 / Published: 2 July 2021
(This article belongs to the Special Issue Signaling Pathways in Cell Generation and Reprogramming)
Induced pluripotent stem (iPS) cells constitute a perfect tool to study human embryo development processes such as myogenesis, thanks to their ability to differentiate into three germ layers. Currently, many protocols to obtain myogenic cells have been described in the literature. They differ in many aspects, such as media components, including signaling modulators, feeder layer constituents, and duration of culture. In our study, we compared three different myogenic differentiation protocols to verify, side by side, their efficiency. Protocol I was based on embryonic bodies differentiation induction, ITS addition, and selection with adhesion to collagen I type. Protocol II was based on strong myogenic induction at the embryonic bodies step with BIO, forskolin, and bFGF, whereas cells in Protocol III were cultured in monolayers in three special media, leading to WNT activation and TGF-β and BMP signaling inhibition. Myogenic induction was confirmed by the hierarchical expression of myogenic regulatory factors MYF5, MYOD, MYF6 and MYOG, as well as the expression of myotubes markers MYH3 and MYH2, in each protocol. Our results revealed that Protocol III is the most efficient in obtaining myogenic cells. Furthermore, our results indicated that CD56 is not a specific marker for the evaluation of myogenic differentiation. View Full-Text
Keywords: myogenesis; skeletal muscle; differentiation; induced pluripotent stem cells (iPS); myogenic factors; CD56 myogenesis; skeletal muscle; differentiation; induced pluripotent stem cells (iPS); myogenic factors; CD56
Show Figures

Figure 1

MDPI and ACS Style

Ulman, A.; Kot, M.; Skrzypek, K.; Szewczyk, B.; Majka, M. Myogenic Differentiation of iPS Cells Shows Different Efficiency in Simultaneous Comparison of Protocols. Cells 2021, 10, 1671. https://doi.org/10.3390/cells10071671

AMA Style

Ulman A, Kot M, Skrzypek K, Szewczyk B, Majka M. Myogenic Differentiation of iPS Cells Shows Different Efficiency in Simultaneous Comparison of Protocols. Cells. 2021; 10(7):1671. https://doi.org/10.3390/cells10071671

Chicago/Turabian Style

Ulman, Aleksandra, Marta Kot, Klaudia Skrzypek, Barbara Szewczyk, and Marcin Majka. 2021. "Myogenic Differentiation of iPS Cells Shows Different Efficiency in Simultaneous Comparison of Protocols" Cells 10, no. 7: 1671. https://doi.org/10.3390/cells10071671

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop