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Article

Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS

1
Jagiellonian Centre of Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland
2
Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland
3
Pharmacology Department, Jagiellonian University Medical College, Grzegorzecka 16, 31-531 Krakow, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Francisco Rivero
Cells 2021, 10(6), 1403; https://doi.org/10.3390/cells10061403
Received: 6 May 2021 / Revised: 28 May 2021 / Accepted: 2 June 2021 / Published: 6 June 2021
(This article belongs to the Section Cellular Biophysics)
Endothelial inflammation is the hallmark of vascular pathology often proceeding with cardiovascular diseases. Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation. Our results show that LPS-induced LDs in HMEC-1 have a composition depending on LPS-incubation time and their formation requires the presence of serum. Robust endothelial inflammation induced by LPS was linked to LDs composed of highly unsaturated lipids, as well as prostacyclin release. LPS-induced LDs were spatially associated with nanostructural changes in the cell membrane architecture. In summary, LDs formation represents an integral component of endothelial inflammation induced by LPS. View Full-Text
Keywords: lipid droplets; Raman imaging; atomic force microscopy; endothelium; inflammation; lipopolysaccharides lipid droplets; Raman imaging; atomic force microscopy; endothelium; inflammation; lipopolysaccharides
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MDPI and ACS Style

Czamara, K.; Stojak, M.; Pacia, M.Z.; Zieba, A.; Baranska, M.; Chlopicki, S.; Kaczor, A. Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS. Cells 2021, 10, 1403. https://doi.org/10.3390/cells10061403

AMA Style

Czamara K, Stojak M, Pacia MZ, Zieba A, Baranska M, Chlopicki S, Kaczor A. Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS. Cells. 2021; 10(6):1403. https://doi.org/10.3390/cells10061403

Chicago/Turabian Style

Czamara, Krzysztof, Marta Stojak, Marta Z. Pacia, Alicja Zieba, Malgorzata Baranska, Stefan Chlopicki, and Agnieszka Kaczor. 2021. "Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS" Cells 10, no. 6: 1403. https://doi.org/10.3390/cells10061403

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