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Article

Codon Bias Can Determine Sorting of a Potassium Channel Protein

1
Membrane Biophysics, Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany
2
Nebraska Center for Virology, Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
3
Department of Biosciences, University of Milan, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to the manuscript.
Academic Editors: Carmen Valenzuela Miranda, Domenico Tricarico and Alexander E. Kalyuzhny
Cells 2021, 10(5), 1128; https://doi.org/10.3390/cells10051128
Received: 3 March 2021 / Revised: 30 April 2021 / Accepted: 5 May 2021 / Published: 7 May 2021
Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins. View Full-Text
Keywords: codon usage; effect of synonymous codon exchange; membrane protein sorting; dual sorting codon usage; effect of synonymous codon exchange; membrane protein sorting; dual sorting
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MDPI and ACS Style

Engel, A.J.; Kithil, M.; Langhans, M.; Rauh, O.; Cartolano, M.; Van Etten, J.L.; Moroni, A.; Thiel, G. Codon Bias Can Determine Sorting of a Potassium Channel Protein. Cells 2021, 10, 1128. https://doi.org/10.3390/cells10051128

AMA Style

Engel AJ, Kithil M, Langhans M, Rauh O, Cartolano M, Van Etten JL, Moroni A, Thiel G. Codon Bias Can Determine Sorting of a Potassium Channel Protein. Cells. 2021; 10(5):1128. https://doi.org/10.3390/cells10051128

Chicago/Turabian Style

Engel, Anja J., Marina Kithil, Markus Langhans, Oliver Rauh, Matea Cartolano, James L. Van Etten, Anna Moroni, and Gerhard Thiel. 2021. "Codon Bias Can Determine Sorting of a Potassium Channel Protein" Cells 10, no. 5: 1128. https://doi.org/10.3390/cells10051128

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