Next Article in Journal
The Discovery of Naringenin as Endolysosomal Two-Pore Channel Inhibitor and Its Emerging Role in SARS-CoV-2 Infection
Previous Article in Journal
Post-Transcriptional Regulation of Viral RNA through Epitranscriptional Modification
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
Cells
Volume 10
Issue 5
10.3390/cells10051128
cells-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement question_answer thumb_up
...
textsms
...
Article

Codon Bias Can Determine Sorting of a Potassium Channel Protein

by
Anja J. Engel
1,†,
Marina Kithil
1,†,
Markus Langhans
1,
Oliver Rauh
1,
Matea Cartolano
1,
James L. Van Etten
2,
Anna Moroni
3 and
Gerhard Thiel
1,*
1
Membrane Biophysics, Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany
2
Nebraska Center for Virology, Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
3
Department of Biosciences, University of Milan, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to the manuscript.
Academic Editors: Carmen Valenzuela Miranda, Domenico Tricarico and Alexander E. Kalyuzhny
Cells 2021, 10(5), 1128; https://doi.org/10.3390/cells10051128
Received: 3 March 2021 / Revised: 30 April 2021 / Accepted: 5 May 2021 / Published: 7 May 2021
View Full-Text Download PDF
Browse Figures
Review Reports

Abstract

Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins. View Full-Text
Keywords: codon usage; effect of synonymous codon exchange; membrane protein sorting; dual sorting codon usage; effect of synonymous codon exchange; membrane protein sorting; dual sorting
►▼ Show Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

SciFeed

Share and Cite

MDPI and ACS Style

Engel, A.J.; Kithil, M.; Langhans, M.; Rauh, O.; Cartolano, M.; Van Etten, J.L.; Moroni, A.; Thiel, G. Codon Bias Can Determine Sorting of a Potassium Channel Protein. Cells 2021, 10, 1128. https://doi.org/10.3390/cells10051128

AMA Style

Engel AJ, Kithil M, Langhans M, Rauh O, Cartolano M, Van Etten JL, Moroni A, Thiel G. Codon Bias Can Determine Sorting of a Potassium Channel Protein. Cells. 2021; 10(5):1128. https://doi.org/10.3390/cells10051128

Chicago/Turabian Style

Engel, Anja J., Marina Kithil, Markus Langhans, Oliver Rauh, Matea Cartolano, James L. Van Etten, Anna Moroni, and Gerhard Thiel. 2021. "Codon Bias Can Determine Sorting of a Potassium Channel Protein" Cells 10, no. 5: 1128. https://doi.org/10.3390/cells10051128

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop