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Article

A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections

1
Department of Oral Medicine and Stomatology, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan
2
Department of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, 3-18-15, Kuramoto, Tokushima 770-8504, Japan
3
Department of Nutrition and Health Promotion, Hiroshima Jogakuin University, 4-13-1, Ushitahigashi, Higashiku, Hiroshima 732-0063, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Ton J. Rabelink and Alexander E. Kalyuzhny
Cells 2021, 10(11), 2827; https://doi.org/10.3390/cells10112827
Received: 27 September 2021 / Revised: 15 October 2021 / Accepted: 19 October 2021 / Published: 21 October 2021
(This article belongs to the Special Issue Dental Pulp Stem Cells and Regenerative Medicine)
Considering that every tissue/organ has the most suitable microenvironment for its functional cells, controlling induced pluripotent stem cell (iPSC) differentiation by culture on frozen sections having a suitable microenvironment is possible. Induced PSCs were cultured on frozen sections of the liver, the brain, the spinal cord, and cover glasses (control) for 9 days. The iPSCs cultured on the sections of the liver resembled hepatocytes, whereas those on sections of the brain and the spinal cord resembled neuronal cells. The percentage of hepatocytic marker-positive cells in the iPSCs cultured on the sections of the liver was statistically higher than that of those in the iPSCs cultured on the sections of the brain and the spinal cord or on cover glasses. In contrast, the iPSCs cultured on the sections of the brain and the spinal cord revealed a high percentage of neural marker-positive cells. Thus, iPSCs can be differentiated into a specific cell lineage in response to specific factors within frozen sections of tissues/organs. Differentiation efficacy of the frozen sections markedly differed between the iPSC clones. Therefore, our induction method could be simple and effective for evaluating the iPSC quality. View Full-Text
Keywords: iPSC; differentiation; frozen section iPSC; differentiation; frozen section
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MDPI and ACS Style

Tadokoro, S.; Tokuyama-Toda, R.; Tatehara, S.; Ide, S.; Umeki, H.; Miyoshi, K.; Noma, T.; Satomura, K. A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections. Cells 2021, 10, 2827. https://doi.org/10.3390/cells10112827

AMA Style

Tadokoro S, Tokuyama-Toda R, Tatehara S, Ide S, Umeki H, Miyoshi K, Noma T, Satomura K. A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections. Cells. 2021; 10(11):2827. https://doi.org/10.3390/cells10112827

Chicago/Turabian Style

Tadokoro, Susumu, Reiko Tokuyama-Toda, Seiko Tatehara, Shinji Ide, Hirochika Umeki, Keiko Miyoshi, Takafumi Noma, and Kazuhito Satomura. 2021. "A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections" Cells 10, no. 11: 2827. https://doi.org/10.3390/cells10112827

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