CRMP2 Is Involved in Regulation of Mitochondrial Morphology and Motility in Neurons
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Isolation and Purification of Brain Mitochondria
2.3. Cell Culture
2.4. Immunocytochemistry
2.5. Alkali and Trypsin Treatment
2.6. Immunoblotting
2.7. Co-Immunoprecipitation
2.8. Neuronal Transfection
2.9. Mitochondrial Morphology
2.10. Mitochondrial Motility
2.11. Statistics
3. Results
3.1. CRMP2 Localization on Mitochondria
3.2. Manipulations with CRMP2 Phosphorylation
3.3. CRMP2 and Proteins Involved in Regulating Mitochondrial Morphology and Motility
3.4. CRMP2 Phosphorylation State and Alterations in Mitochondrial Morphology and Motility
3.5. CRMP2 Deletion Correlates with Alterations in Mitochondrial Morphology and Motility
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Brustovetsky, T.; Khanna, R.; Brustovetsky, N. CRMP2 Is Involved in Regulation of Mitochondrial Morphology and Motility in Neurons. Cells 2021, 10, 2781. https://doi.org/10.3390/cells10102781
Brustovetsky T, Khanna R, Brustovetsky N. CRMP2 Is Involved in Regulation of Mitochondrial Morphology and Motility in Neurons. Cells. 2021; 10(10):2781. https://doi.org/10.3390/cells10102781
Chicago/Turabian StyleBrustovetsky, Tatiana, Rajesh Khanna, and Nickolay Brustovetsky. 2021. "CRMP2 Is Involved in Regulation of Mitochondrial Morphology and Motility in Neurons" Cells 10, no. 10: 2781. https://doi.org/10.3390/cells10102781