Role of MiR-325-3p in the Regulation of CFL2 and Myogenic Differentiation of C2C12 Myoblasts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture, Differentiation and PA Treatment
2.2. Transfection of Oligonucleotides
2.3. RNA Extraction, PCR and Quantitative Real-Time PCR (qRT-PCR)
2.4. Dual-Luciferase Assay
2.5. Immunoblot Analysis
2.6. Immunofluorescence Analysis
2.7. Cell Proliferation Assays
2.8. Flow Cytometry
2.9. Database and Statistical Analysis
3. Results
3.1. PA Inhibited Myogenic Differentiation but Elevated miR-325-3p Expression
3.2. MiR-325-3p Directly Targeted CFL2 3′UTR
3.3. MiR-325-3p Increased F-Actin and Nuclear Yes-Associated Protein (YAP)
3.4. MiR-325-3p Promoted Myoblast Proliferation
3.5. MiR-325-3p Suppressed the Expressions of Myogenic Factors
3.6. MiR-325-3p Impeded Myogenic Differentiation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Nguyen, M.T.; Lee, W. Role of MiR-325-3p in the Regulation of CFL2 and Myogenic Differentiation of C2C12 Myoblasts. Cells 2021, 10, 2725. https://doi.org/10.3390/cells10102725
Nguyen MT, Lee W. Role of MiR-325-3p in the Regulation of CFL2 and Myogenic Differentiation of C2C12 Myoblasts. Cells. 2021; 10(10):2725. https://doi.org/10.3390/cells10102725
Chicago/Turabian StyleNguyen, Mai Thi, and Wan Lee. 2021. "Role of MiR-325-3p in the Regulation of CFL2 and Myogenic Differentiation of C2C12 Myoblasts" Cells 10, no. 10: 2725. https://doi.org/10.3390/cells10102725