CDK1-Mediated Phosphorylation of BAG3 Promotes Mitotic Cell Shape Remodeling and the Molecular Assembly of Mitotic p62 Bodies
Abstract
:1. Introduction
2. Materials and Methods
2.1. Expression Vectors, Recombinant Adenoviruses, and Baculoviruses
- BAG3S284-T285A.gen: 5′-CCA GCC AGG AGC GCC GCG CCA CTC CAC TCC-3′;
- BAG3S284-T285A.rev: 5′-GGA GTG GAG TGG CGC GGC GCT CCT GGC TGG-3′;
- BAG3T285D.gen: 5′-CCA GCC AGG AGC AGCGAC CCA CTC CAC TCC-3′;
- BAG3T285D.rev: 5′-GGA GTG GAG TGG GTC GCT GCT CCT GGC TGG-3′;
- BAG3S289A.gen: 5′-CGCCACTCCACGCCCCCTCGCCCATC-3′;
- BAG3S289A.rev: 5′-GATGGGCGAGGGGGCGTGGAGTGGCG-3′;
- BAG3S291A.gen: 5′-CTCCACTCCCCCGCGCCCATCCGTGTG-3′;
- BAG3S291A.gen: 5′-CACACGGATGGGCGCGGGGGAGTGGAG-3′.
2.2. Antibodies and Chemicals
2.3. Cell Lines, Cell Culture, and Cell Synchronization
2.4. In Vitro Kinase Assay
2.5. Knockdown-Rescue Experiments, RNA interference, and Biochemistry
siCtl allStars | 5′-CAGGGTATCGACGATTACAAA-3′ |
siBAG3 (ORF) | 5′-CGAAGAGTATTTGACCAAA-3′; formerly described as siBAG3_1 [6] |
siBAG3(3′UTR_1) | 5′-GATGTGTGCTTTAGGGAAT-3′; formerly described as siBAG3_3 [6] |
siBAG3(3′UTR_2) | 5′-CTGACTTTAGAGAGAGTAATT-3′ |
siHSPB8_1 | 5′-CAGAGGAGTTGATGGTGAA-3′; formerly described as siHSPB8_3 [6] |
siHSPB8_2 | 5′-GCAGTGAATGCAAGGGTTATT-3′ [6] |
sip62_1 | 5′-GGAAATGGGTCCACCAGGA-3′ [6] |
sip62_2 | 5′-AGACCAAGAACTATGACAT-3′ [6] |
2.6. Immunofluorescence, Microscopy, and Live-Cell Imaging
2.7. Statistical Analyses
3. Results
3.1. BAG3 Is Phosphorylated at T285 at Mitotic Entry
3.2. BAG3 Phosphorylation at T285 Is Regulated by CDK1
3.3. BAG3 Phosphorylation Dynamics at S284-T285 Regulate Mitotic Cell Shape Remodeling
3.4. BAG3 Mitotic Phosphorylation Is Regulated by p62
3.5. BAG3 Phosphorylation and HSPB8 Regulate the Molecular Assembly of p62 Bodies during Mitosis
4. Discussion
Supplementary Materials
Supplemental Methods: Mass Spectrometry and Phospho-Peptides Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Luthold, C.; Lambert, H.; Guilbert, S.M.; Rodrigue, M.-A.; Fuchs, M.; Varlet, A.-A.; Fradet-Turcotte, A.; Lavoie, J.N. CDK1-Mediated Phosphorylation of BAG3 Promotes Mitotic Cell Shape Remodeling and the Molecular Assembly of Mitotic p62 Bodies. Cells 2021, 10, 2638. https://doi.org/10.3390/cells10102638
Luthold C, Lambert H, Guilbert SM, Rodrigue M-A, Fuchs M, Varlet A-A, Fradet-Turcotte A, Lavoie JN. CDK1-Mediated Phosphorylation of BAG3 Promotes Mitotic Cell Shape Remodeling and the Molecular Assembly of Mitotic p62 Bodies. Cells. 2021; 10(10):2638. https://doi.org/10.3390/cells10102638
Chicago/Turabian StyleLuthold, Carole, Herman Lambert, Solenn M. Guilbert, Marc-Antoine Rodrigue, Margit Fuchs, Alice-Anaïs Varlet, Amélie Fradet-Turcotte, and Josée N. Lavoie. 2021. "CDK1-Mediated Phosphorylation of BAG3 Promotes Mitotic Cell Shape Remodeling and the Molecular Assembly of Mitotic p62 Bodies" Cells 10, no. 10: 2638. https://doi.org/10.3390/cells10102638