Methodical Nitrogen–Water Distribution System Enhances Rice Yield While Reducing Environmental Losses: Evidence from 15N Isotope Tracing
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Design Framework
2.2. Treatment Implementation and Management Protocols
2.3. Integrated Micro-Plot System for 15N Tracing
2.4. Environmental Loss Monitoring Systems
2.5. Greenhouse Gas Emission Monitoring
2.6. Yield Assessment and Tissue Analysis
2.7. Analytical Methods and Statistical Framework
3. Results
3.1. Effects of Water–Nitrogen Management on Rice Yield and Yield Components
3.2. Effects of Water–Nitrogen Management on Rice Tillering Dynamics
3.3. Nitrogen Accumulation and Partitioning
3.4. Fate of Nitrogen in Paddy Fields
3.4.1. Residual Differences of Fertilizer-Derived Nitrogen in Soil
3.4.2. Fertilizer-Derived Nitrogen Losses via Runoff and Leaching
3.4.3. N2O Emissions
3.4.4. 15N Balance and Fate
3.5. Integrated Analysis of Nitrogen Fate and Yield Performance
4. Discussion
4.1. Physiological and Spatial Mechanisms Underlying Yield Enhancement with Reduced Nitrogen Input
4.2. Environmental Benefits Through Nitrogen Cycling Optimization
4.3. Application Challenges and Implementation Strategies of MNWD Technology in Small-Scale Agriculture
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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| Year | Depth (cm) | pH | Organic Matter (g kg−1) | Total N (g kg−1) | Available N (mg kg−1) | Available P (mg kg−1) | Available K (mg kg−1) |
|---|---|---|---|---|---|---|---|
| 2019 | 0~10 | 5.91 | 22.94 | 1.80 | 111.61 | 23.89 | 107.23 |
| 10~20 | 5.83 | 16.12 | 1.06 | 101.72 | 16.61 | 91.86 | |
| 20~30 | 5.51 | 10.98 | 0.95 | 82.85 | 12.12 | 64.72 | |
| 2020 | 0~10 | 6.13 | 24.67 | 1.93 | 123.19 | 25.67 | 122.81 |
| 10~20 | 5.92 | 18.39 | 1.29 | 116.30 | 18.18 | 108.18 | |
| 20~30 | 5.87 | 12.42 | 1.02 | 99.41 | 13.11 | 72.83 |
| Water and Nitrogen Management Mode | Nitrogen Management | Water Management |
|---|---|---|
| FP | Nitrogen fertilizer was applied as urea (46% N) at a total rate of 150 kg N ha−1, partitioned into two applications: 70% (105 kg N ha−1) as basal fertilizer one day before transplanting and 30% (45 kg N ha−1) as tillering fertilizer seven days after transplanting. | Continuous flooding irrigation: A water depth of 3–5 cm was maintained throughout the growing period from transplanting to physiological maturity, except during mid-season drainage at the inactive tillering stage when fields were drained and allowed to dry until hairline cracks appeared on the soil surface. |
| NWC | Nitrogen fertilizer was applied using a leaf age-based split application strategy comprising four discrete applications: basal fertilizer one day before transplanting, tillering fertilizer seven days after transplanting, panicle initiation fertilizer 42 days after transplanting (6.5–7.0 leaf age), and spikelet fertility fertilizer 56 days after transplanting (8.5–9.0 leaf age) to minimize spikelet degeneration during the reproductive stage. The nitrogen distribution followed a 3:3:2:2 ratio (basal:tillering:panicle initiation:spikelet fertility), with a total application rate of 150 kg N ha−1. | Alternate wetting and drying irrigation (AWD): The irrigation regime was implemented through stage-specific water management protocols designed to optimize plant physiological responses while conserving water resources. During the establishment phase (0–10 days after transplanting), a shallow water layer of 2–3 cm was maintained to facilitate seedling recovery and re-establishment following transplant shock. Throughout the active tillering period, irrigation management followed a systematic wet–dry cycle: fields were initially flooded to 3–5 cm depth, then allowed to drain naturally until soil water potential reached −20 kPa (monitored using NJ-1 tensiometers [Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China] installed at 15–20 cm depth). These tensiometers feature a ceramic probe with stainless steel casing, operating within a 0–85 kPa measurement range, ensuring accurate monitoring of soil moisture thresholds. Re-irrigation was applied when the threshold was reached to restore the target water depth. This alternating cycle continued until the inactive tillering stage, when prolonged drainage was implemented to achieve soil water potential below −30 kPa, creating stress conditions conducive to suppressing non-productive tillers. During the reproductive phase (booting to heading), a continuous water layer of 3–5 cm was maintained without drainage interruption to ensure optimal panicle development and prevent spikelet abortion under water stress. The grain-filling period employed a modified AWD protocol wherein fields were initially flooded to 5–8 cm depth to support early grain development, followed by controlled drying cycles allowing soil water potential to decrease to −25 kPa before re-irrigation. This wet–dry alternation continued until final drainage approximately seven days before harvest to facilitate grain maturation and enable mechanical harvesting operations. |
| MNWD | For the uniform nitrogen distribution regime, nitrogen fertilizer was applied according to a systematic seven-split schedule designed to maintain consistent nutrient availability throughout the growing period. Applications were made at 7, 14, 35, 49, 56, 70, and 77 days after transplanting (DAT), corresponding to early tillering, active tillering, stem elongation, panicle initiation, booting, heading, and grain filling stages, respectively. The application rates were 30 kg N ha−1 for the initial application (7 DAT) and 15 kg N ha−1 for each subsequent application (14, 35, 49, 56, 70, and 77 DAT), yielding a total nitrogen input of 120 kg N ha−1. | Synchronized fertigation with uniform soil moisture management: This integrated water–fertilizer management system was designed to maintain optimal soil moisture conditions while ensuring precise nutrient delivery throughout the growing season. Following transplanting, a shallow water layer of 2–3 cm was established to facilitate seedling establishment and root development. The fertigation protocol employed real-time soil moisture monitoring coupled with synchronized nutrient application. During scheduled fertilizer applications, irrigation management followed a dual-threshold strategy: when an existing water layer was present, supplemental irrigation of 2 cm depth was applied to ensure adequate nutrient dissolution and distribution; when fields were at field capacity without standing water, irrigation continued until soil saturation was achieved to create optimal conditions for nutrient infiltration and root uptake. This approach ensured uniform fertilizer distribution while minimizing surface runoff and volatilization losses. During inter-fertilization periods, the soil water potential was continuously monitored using the same NJ-1 tensiometers [Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China] at 15–20 cm depth, with irrigation triggered when soil water potential declined to −15 kPa. This threshold maintained near-optimal soil moisture conditions, preventing water stress while avoiding anaerobic conditions that could impair root function and nutrient uptake efficiency. Irrigation was terminated approximately seven days before harvest to facilitate grain desiccation and optimize field conditions for mechanical harvesting. (The MNWD system relies solely on conventional field irrigation channels along rice planting rows to deliver diluted nitrogen fertilizer solution, without dedicated fertilizer distribution pipes.) |
| Year | Water–Nitrogen Management | Panicle Number (×104 ha−1) | Spikelets per Panicle | Spikelet Fertility (%) | 1000-Grain Weight (g) | Total Spikelets (×106 ha−1) | Grain Yield (kg ha−1) |
|---|---|---|---|---|---|---|---|
| 2019 | FP | 154.53 ± 4.85 a | 154.17 ± 2.75 b | 90.85 ± 0.47 b | 36.15 ± 0.05 b | 238.19 ± 6.04 b | 7419 ± 127 b |
| NWC | 156.36 ± 6.06 a | 161.05 ± 6.00 a | 90.92 ± 1.35 b | 36.47 ± 0.08 a | 251.64 ± 2.91 a | 7889 ± 179 a | |
| MNWD | 161.76 ± 2.58 a | 155.01 ± 2.08 b | 92.67 ± 1.52 a | 36.48 ± 0.05 a | 250.74 ± 4.64 a | 8087 ± 152 a | |
| 2020 | FP | 188.10 ± 6.48 a | 156.61 ± 0.82 b | 85.83 ± 0.62 c | 36.18 ± 0.02 b | 294.58 ± 10.51 b | 8896 ± 188 c |
| NWC | 194.04 ± 6.84 a | 174.47 ± 3.66 a | 86.40 ± 0.52 c | 36.22 ± 0.05 b | 338.41 ± 6.82 a | 9727 ± 164 b | |
| MNWD | 194.46 ± 0.18 a | 173.63 ± 1.25 a | 89.61 ± 0.40 b | 36.37 ± 0.02 a | 337.65 ± 2.63 a | 10,284 ± 178 a |
| Project | Water–Nitrogen Management | Nitrogen Accumulation in Shoot at Maturity Stage (kg ha−1) | |
|---|---|---|---|
| 2019 | 2020 | ||
| TN (kg ha−1) | FP | 112.80 ± 3.32 c | 140.34 ± 1.77 c |
| NWC | 140.36 ± 2.49 a | 172.99 ± 1.78 a | |
| MNWD | 132.73 ± 2.28 b | 165.08 ± 2.32 b | |
| 15N (kg ha−1) | FP | 48.17 ± 1.85 c | 46.65 ± 1.21 c |
| NWC | 71.15 ± 2.15 a | 76.77 ± 2.68 a | |
| MNWD | 63.50 ± 1.58 b | 67.96 ± 2.91 b | |
| Soil nitrogen dependency (%) | FP | 57.30 ± 1.89 a | 66.76 ± 1.89 a |
| NWC | 49.31 ± 1.59 c | 55.62 ± 1.26 c | |
| MNWD | 52.16 ± 0.72 b | 58.83 ± 1.11 b | |
| Nitrogen fertilizer recovery (%) | FP | 32.11 ± 1.23 c | 31.10 ± 0.94 c |
| NWC | 47.43 ± 1.43 b | 51.18 ± 1.73 b | |
| MNWD | 52.92 ± 1.32 a | 56.63 ± 1.44 a | |
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Yang, Z.; Li, Y.; Shi, Y.; Xie, H.; Liu, B.; Shu, C.; Cheng, Q.; Chen, S.; Wang, L.; Chen, Q.; et al. Methodical Nitrogen–Water Distribution System Enhances Rice Yield While Reducing Environmental Losses: Evidence from 15N Isotope Tracing. Agronomy 2026, 16, 801. https://doi.org/10.3390/agronomy16080801
Yang Z, Li Y, Shi Y, Xie H, Liu B, Shu C, Cheng Q, Chen S, Wang L, Chen Q, et al. Methodical Nitrogen–Water Distribution System Enhances Rice Yield While Reducing Environmental Losses: Evidence from 15N Isotope Tracing. Agronomy. 2026; 16(8):801. https://doi.org/10.3390/agronomy16080801
Chicago/Turabian StyleYang, Zhiyuan, Yu Li, Yuanqing Shi, Hongkun Xie, Binbin Liu, Chuanhai Shu, Qingyue Cheng, Song Chen, Lanpeng Wang, Qiqi Chen, and et al. 2026. "Methodical Nitrogen–Water Distribution System Enhances Rice Yield While Reducing Environmental Losses: Evidence from 15N Isotope Tracing" Agronomy 16, no. 8: 801. https://doi.org/10.3390/agronomy16080801
APA StyleYang, Z., Li, Y., Shi, Y., Xie, H., Liu, B., Shu, C., Cheng, Q., Chen, S., Wang, L., Chen, Q., Liuru, H., Peng, Z., Chen, Z., Ma, J., Sun, Y., & Li, N. (2026). Methodical Nitrogen–Water Distribution System Enhances Rice Yield While Reducing Environmental Losses: Evidence from 15N Isotope Tracing. Agronomy, 16(8), 801. https://doi.org/10.3390/agronomy16080801

