Next Article in Journal
National-Scale Assessment of Soil pH Change in Chinese Croplands from 1980 to 2018
Previous Article in Journal
Transcriptome and Candidate Gene Analysis of the Seed Germination Rate Gene in Capsicum
Previous Article in Special Issue
Effects of SiO2 Nanoparticles on the Yield and Quality of Sophora tonkinensis Under Drought Stress
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Agronomy
Volume 15
Issue 12
10.3390/agronomy15122773
agronomy-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Comparative Immunomodulatory Efficacy of Chemogenic and Biogenic Manganese Nanoparticles for Inducing Arsenic Stress Resilience in Rapeseed

by
Muhammad Arslan Yousaf
1,
Muhammad Noman
2,3,
Ayesha Khalil Maan
4,
Basharat Ali
5,
Muhammad Kamran
6,
Muhammad Shahbaz Naeem
7,
Mohammad Shafiqul Islam
8,
Yiwa Hu
1,
Skhawat Ali
1,* and
Weijun Zhou
1,*
1
Institute of Crop Science, Ministry of Agriculture and Rural Affairs Key Laboratory of Spectroscopy Sensing, Zhejiang University, Hangzhou 310058, China
2
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China
3
Department of Plant Biotechnology, Korea University, Seoul 02481, Republic of Korea
4
Department of Chemistry, University of Agriculture, Faisalabad 38000, Pakistan
5
Department of Agricultural Engineering, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan 64200, Pakistan
6
Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
7
Department of Agronomy, University of Agriculture, Faisalabad 38000, Pakistan
8
State Key Laboratory of Agricultural Products Safety, Key Laboratory of Agricultural Microbiome of Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
*
Authors to whom correspondence should be addressed.
Agronomy 2025, 15(12), 2773; https://doi.org/10.3390/agronomy15122773 (registering DOI)
Submission received: 25 October 2025 / Revised: 26 November 2025 / Accepted: 28 November 2025 / Published: 30 November 2025
(This article belongs to the Special Issue Regulation of Nanomaterials in Crop Growth and Physiology Under Abiotic Stress)
Versions Notes

Abstract

Arsenic (As) contamination poses a critical threat to agricultural productivity, affecting rapeseed (Brassica napus L.), an agronomically important crop. A comparative assessment was performed to evaluate the efficacy of chemogenic and biogenic manganese nanoparticles (C-MnNPs and B-MnNPs) for mitigating As toxicity. B-MnNPs were biosynthesized using cell-free filtrate of Bacillus pumilus MAY4, while C-MnNPs were obtained from Cwnano Co., Ltd. (Shanghai, China). Greenhouse assays demonstrated that both C-MnNPs and B-MnNPs alleviated detrimental effects of As; however, B-MnNPs exhibited superior performance compared to their chemical counterparts. Compared to As-stressed plants, B-MnNPs enhanced leaf and root biomass (26.4% and 56.15%, respectively), net photosynthetic rate (64.8%), and stomatal conductance (50%). B-MnNPs more effectively reduced oxidative stress markers by activating antioxidant defense systems in both leaf and root tissues. Furthermore, B-MnNPs reduced in planta As accumulation while significantly improving uptake of essential nutrients, including potassium, phosphorous, magnesium, and manganese, etc., in rapeseed plants. Expression studies revealed that B-MnNPs upregulated antioxidant defense and redox homeostasis related stress-responsive genes under induced As stress. Biochemical assays further confirmed the enrichment of stress-responsive phytohormones, including salicylic acid, jasmonic acid, and abscisic acid, in B-MnNP-treated As-stressed rapeseed plants, indicating activation of multi-tier defense response by B-MnNPs to cope with As stress. These findings establish B-MnNPs as a highly effective nano-enabled strategy for managing As toxicity in the rapeseed cultivation system. This research provides critical insights into the molecular and physiological mechanisms underlying MnNP-mediated stress tolerance and offers a promising green nanotechnology approach for heavy metal-resilient crops.
Keywords: biogenic nanoparticles; Bacillus pumilus; manganese nanoparticles; arsenic toxicity; Brassica napus; oxidative stress; antioxidant defense; phytohormones biogenic nanoparticles; Bacillus pumilus; manganese nanoparticles; arsenic toxicity; Brassica napus; oxidative stress; antioxidant defense; phytohormones

Share and Cite

MDPI and ACS Style

Yousaf, M.A.; Noman, M.; Maan, A.K.; Ali, B.; Kamran, M.; Naeem, M.S.; Islam, M.S.; Hu, Y.; Ali, S.; Zhou, W. Comparative Immunomodulatory Efficacy of Chemogenic and Biogenic Manganese Nanoparticles for Inducing Arsenic Stress Resilience in Rapeseed. Agronomy 2025, 15, 2773. https://doi.org/10.3390/agronomy15122773

AMA Style

Yousaf MA, Noman M, Maan AK, Ali B, Kamran M, Naeem MS, Islam MS, Hu Y, Ali S, Zhou W. Comparative Immunomodulatory Efficacy of Chemogenic and Biogenic Manganese Nanoparticles for Inducing Arsenic Stress Resilience in Rapeseed. Agronomy. 2025; 15(12):2773. https://doi.org/10.3390/agronomy15122773

Chicago/Turabian Style

Yousaf, Muhammad Arslan, Muhammad Noman, Ayesha Khalil Maan, Basharat Ali, Muhammad Kamran, Muhammad Shahbaz Naeem, Mohammad Shafiqul Islam, Yiwa Hu, Skhawat Ali, and Weijun Zhou. 2025. "Comparative Immunomodulatory Efficacy of Chemogenic and Biogenic Manganese Nanoparticles for Inducing Arsenic Stress Resilience in Rapeseed" Agronomy 15, no. 12: 2773. https://doi.org/10.3390/agronomy15122773

APA Style

Yousaf, M. A., Noman, M., Maan, A. K., Ali, B., Kamran, M., Naeem, M. S., Islam, M. S., Hu, Y., Ali, S., & Zhou, W. (2025). Comparative Immunomodulatory Efficacy of Chemogenic and Biogenic Manganese Nanoparticles for Inducing Arsenic Stress Resilience in Rapeseed. Agronomy, 15(12), 2773. https://doi.org/10.3390/agronomy15122773

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop