Exogenous Application of Salicylic Acid Modulates Oxidative Stress during the Seed Development of Rice (Oryza sativa L.) Grain
Round 1
Reviewer 1 Report
Dear Authors,
See the attached comments below.
Comments for author File: Comments.pdf
Author Response
Please, see file attached.
Author Response File: Author Response.docx
Reviewer 2 Report
Abstract:
The application period of exogenous SA should be added.
1 Introduction
In my opinion, the promise is defective, due to the role of phytoprostanes (PhytoPs), phytofurans (PhytoFs) and oxylipin involved in ROS producing and clearing was not elaborated sufficient.
Line59: …21% higher than……
2. Materials and Methods
Line 120. Why choice the three cultivars? What is the different between the three cultivars aim to the study content. Although the cultivar difference described in the 3.1., I suggest to make a direct statement in 2.2.
3 Results and discussion
I have no objection to the results of the study, but the result only explained the differences among cultivars, and did not explain enough about the mechanism of how ROS and oxylipin affect grain filling, and how the PhytoPs and PhytoFs participate in the regulation. I think this is the most innovation rather than the cultivar difference. I suggested to improve the content in the results and discussion.
3.4. The value of grain weight should be added, because the study aimed to the period of grain filling.
Author Response
Please, see file attached.
Author Response File: Author Response.docx
Reviewer 3 Report
The manuscript presents a nice data set on (Exogenous Application of Salicylic Acid Modulates Oxidative Stress During the seed development of Rice (Oryza sativa L.) Grain).
The reviewed manuscript entitled “Exogenous Application of Salicylic Acid Modulates Oxidative Stress During the seed development of Rice (Oryza sativa L.) Grain” is generally clear and well written, apart from lack of clarity in some parts, and some specifics. This study is focused on the effect of exogenously foliar applied of salicylic acid (SA) in the concentration 0, 1 and 15 mM, on the oxidative stress.
However, I think this manuscript can only be published after addressing several minor comments as following:
1. Introduction part: Must contains the whole background regarding the targeted problem and how to solve that problem with comparison with literature review; please check and revised accordingly.
2. Materials and methods section:
Must contains recent and related references with more details to be beneficial to broad scientific readers.
3. Results: Figures not clear. The quality of Figures should be improved.
4. Despite the importance of the study and its focus on Modulates Oxidative Stress, salicylic acid plays other roles in other physiological processes, such as photosynthesis, and the measurement of plant hormones should have been measured.
5. Discussion part needs to recheck since no deep discussion with related studies had been mentioned.
6. Conclusion part must contain the importance of paper, the future work and novel
Author Response
Please, see file attached
Author Response File: Author Response.docx
Round 2
Reviewer 2 Report
The comments of the last review have not been well revised. There are three main deficiencies.
1 Introduction
In my opinion, the promise is defective, due to the role of phytoprostanes (PhytoPs), phytofurans (PhytoFs) and oxylipin involved in ROS producing and clearing was not elaborated sufficient.
3 Results and discussion
I have no objection to the results of the study, but the result only explained the differences among cultivars, and did not explain enough about the mechanism of how ROS and oxylipin affect grain filling, and how the PhytoPs and PhytoFs participate in the regulation. I think this is the most innovation rather than the cultivar difference. I suggested to improve the content in the results and discussion.
3.4. The value of grain weight should be added, because the study aimed to the period of grain filling.
Author Response
Please see comments attached.