Rapid Nondestructive Detection of Chlorophyll Content in Muskmelon Leaves under Different Light Quality Treatments
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Materials
2.2. Experimental Design
2.3. Spectral Data Acquisition
2.3.1. Hyperspectral Imaging Equipment and Image Calibration
2.3.2. Region of Interest Selection and Sample Division
2.4. Spectral Data Analysis
2.4.1. Spectral Data Preprocessing
2.4.2. Extraction of Characteristic Wavelengths
2.4.3. Model Building and Evaluation
3. Results
3.1. Spectral Data Acquisition
3.2. Analysis of Pretreatment Effect
3.3. Modeling Based on Characteristic Wavelengths
3.3.1. Feature Wavelength Extraction
3.3.2. PLSR model of Characteristic Wavelengths
3.4. Comparative Analysis of Different Building Models
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Handle | Light Ratio | Light Quantum Flux (μmol/(m2·s)) | Photoperiod (h) |
---|---|---|---|
T1 | 3R/2B/3W | 360 | 12 |
T2 | 8R/4B/5W/2FR/1UVa | ||
T3 | 6R/1B/2W | ||
T4 | 4R/3B/2W/1FR | ||
T5 | 7R/3B/5W/1UVa | ||
CK | White light |
Type | PCs | Rc | RMSEC (mg/g) | Rcv | RMSECV (mg/g) | Rp | RMSEP (mg/g) |
---|---|---|---|---|---|---|---|
Raw | 12 | 0.847 | 1.881 | 0.786 | 2.205 | 0.807 | 2.056 |
Gaussian Filter | 6 | 0.823 | 2.012 | 0.794 | 2.154 | 0.807 | 2.056 |
S-G | 15 | 0.860 | 1.806 | 0.790 | 2.161 | 0.790 | 2.395 |
MSC | 11 | 0.835 | 1.948 | 0.758 | 2.333 | 0.790 | 2.144 |
SNV | 6 | 0.819 | 2.029 | 0.779 | 2.225 | 0.819 | 2.029 |
Detrending | 13 | 0.857 | 1.824 | 0.750 | 2.388 | 0.776 | 2.221 |
Type | PCs | RC | RMSEC (mg/g) | RCV | RMSECV (mg/g) | RP | RMSEP (mg/g) |
---|---|---|---|---|---|---|---|
SPA | 7 | 0.826 | 1.991 | 0.804 | 2.103 | 0.789 | 2.154 |
CARS | 8 | 0.821 | 2.020 | 0.794 | 2.152 | 0.797 | 2.108 |
VCPA | 9 | 0.844 | 1.897 | 0.817 | 2.045 | 0.824 | 1.973 |
UVE | 8 | 0.755 | 2.321 | 0.707 | 2.510 | 0.700 | 2.671 |
GAPLS | 5 | 0.703 | 2.931 | 0.793 | 2.995 | 0.760 | 2.671 |
iVISSA | 9 | 0.840 | 1.918 | 0.800 | 2.119 | 0.813 | 2.125 |
Spectral Feature Extraction Method | RC | RMSEC (mg/g) | RP | RMSEP (mg/g) |
---|---|---|---|---|
PLSR | 0.844 | 1.897 | 0.824 | 1.973 |
LSSVM | 0.819 | 1.997 | 0.799 | 2.214 |
CNN | 0.915 | 1.445 | 0.811 | 2.055 |
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Ma, L.; Zhang, Y.; Zhang, Y.; Wang, J.; Li, J.; Gao, Y.; Wang, X.; Wu, L. Rapid Nondestructive Detection of Chlorophyll Content in Muskmelon Leaves under Different Light Quality Treatments. Agronomy 2022, 12, 3223. https://doi.org/10.3390/agronomy12123223
Ma L, Zhang Y, Zhang Y, Wang J, Li J, Gao Y, Wang X, Wu L. Rapid Nondestructive Detection of Chlorophyll Content in Muskmelon Leaves under Different Light Quality Treatments. Agronomy. 2022; 12(12):3223. https://doi.org/10.3390/agronomy12123223
Chicago/Turabian StyleMa, Ling, Yao Zhang, Yiyang Zhang, Jing Wang, Jianshe Li, Yanming Gao, Xiaomin Wang, and Longguo Wu. 2022. "Rapid Nondestructive Detection of Chlorophyll Content in Muskmelon Leaves under Different Light Quality Treatments" Agronomy 12, no. 12: 3223. https://doi.org/10.3390/agronomy12123223